JMJ17-WRKY40 and HY5-ABI5 modules regulate the expression of ABA-responsive genes in Arabidopsis
JMJ17-WRKY40 and HY5-ABI5 modules regulate the expression of ABA-responsive genes in Arabidopsis
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DOI：
10.1111/nph.17177
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发表时间：
2021-02-12
期刊：
影响因子：
9.4
通讯作者：
Xu, Zheng-Yi
中科院分区：
文献类型：
--
作者：
Wang, Tian-Jing;Huang, Shuangzhan;Xu, Zheng-Yi
脱落酸（阿坝）在幼苗对环境胁迫的适应中起着至关重要的作用。然而，阿坝对种子萌发和幼苗生长的影响中的表观遗传成分和核心转录机制的作用仍然不清楚，在这里，我们表明，组蛋白3赖氨酸4（H3 K4）去甲基化酶，JMJ 17，调节ABA响应基因的表达在种子萌发和幼苗生长。使用比较interactomics，WRKY 40，在阿坝信号传导的中央转录抑制子，被证明与JMJ 17相互作用。WRKY 40促进JMJ 17向ABI 5染色质的募集，其从ABI 5染色质去除基因激活标记（H3 K4 me 3），从而抑制其表达。此外，WRKY 40抑制HY 5的转录激活活性，HY 5可以通过直接结合其启动子来激活ABI 5表达。阿坝浓度的增加会降低WRKY 40对ABI 5启动子的亲和力。因此，WRKY 40和JMJ 17从ABI 5染色质释放，激活HY 5。我们的研究结果揭示了一个新的转录开关，由JMJ 17-WRKY 40和HY 5-ABI 5模块组成，在拟南芥种子萌发和幼苗发育过程中调节阿坝反应。
Abscisic acid (ABA) plays a crucial role in the adaptation of young seedlings to environmental stresses. However, the role of epigenetic components and core transcriptional machineries in the effect of ABA on seed germination and seedling growth remain unclear.Here, we show that a histone 3 lysine 4 (H3K4) demethylase, JMJ17, regulates the expression of ABA-responsive genes during seed germination and seedling growth. Using comparative interactomics, WRKY40, a central transcriptional repressor in ABA signaling, was shown to interact with JMJ17. WRKY40 facilitates the recruitment of JMJ17 to the ABI5 chromatin, which removes gene activation marks (H3K4me3) from the ABI5 chromatin, thereby repressing its expression.Additionally, WRKY40 represses the transcriptional activation activity of HY5, which can activate ABI5 expression by directly binding to its promoter. An increase in ABA concentrations decreases the affinity of WRKY40 for the ABI5 promoter. Thus, WRKY40 and JMJ17 are released from the ABI5 chromatin, activating HY5. The accumulated ABI5 protein further shows heteromeric interaction with HY5, and thus synergistically activates its own expression.Our findings reveal a novel transcriptional switch, composed of JMJ17-WRKY40 and HY5-ABI5 modules, which regulates the ABA response during seed germination and seedling development in Arabidopsis.