Tandem mass tag-based proteomics technology provides insights into multi-targeted mechanism of peptide MOp2 from Moringa oleifera seeds against Staphylococcus aureus
Tandem mass tag-based proteomics technology provides insights into multi-targeted mechanism of peptide MOp2 from Moringa oleifera seeds against Staphylococcus aureus
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DOI：
10.1016/j.lwt.2023.114617
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发表时间：
2023-03-07
影响因子：
6
通讯作者：
Wang，Xuefeng
中科院分区：
文献类型：
--
作者：
Huang，Zhiyuan;Dong，Wenming;Wang，Xuefeng
Our previous study proved that the peptide MOp2 fromMoringa oleiferaseeds exhibited membrane-damaging effects onStaphylococcus aureus. In this study, TMT-based proteomics technology was mainly used to investigate the antimicrobial mechanism of MOp2 againstS. aureus. A total of 541 differentially expressed proteins (DEPs) were identified inS. aureustreated with MOp2, among which 256 and 285 DEPs were upregulated and downregulated, respectively. They were mainly involved in inositol phosphate metabolism,S. aureusinfection, citrate cycle (TCA cycle), and phosphotransferase system (PTS) and acted as ABC transporters and ribosomes.Moreover, the decreasing AKP activity indicated that MOp2 affected cell wall biosynthesis, and excessive ROS accumulation caused apoptosis, resulting in DNA fragmentation, nuclear morphological changes, and phosphatidylserine externalization. Additionally, molecular docking showed that MOp2 could interact with eight DEPs, including ProC, QoxB, SOD2, DnaK, GroEL, RplY, AcpS, and FabG, indicating that MOp2 might act on these molecular targets, leading to increased cell permeability, oxidative damage, impaired protein synthesis, cell wall synthesis obstruction, and energy metabolism disorder. Overall, these findings provided new insights into the multi-targeted mechanism of MOp2 againstS. aureus, and could provide a theoretical basis for its application as a novel food preservative and antibiotic substitute.
