Monoclonal antibodies reactive with mucin expressed in breast cancer
Monoclonal antibodies reactive with mucin expressed in breast cancer
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与乳腺癌中表达的粘蛋白发生反应的单克隆抗体
DOI：
10.1038/icb.1989.29
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发表时间：
1989
影响因子：
4
通讯作者：
I. McKenzie
中科院分区：
文献类型：
--
作者：
P. Xing;J. Tjandra;S. Stacker;JG Teh;C. Thompson;PJ McLaughlin;I. McKenzie
本文报道了三种抗人乳脂球膜（HMFGM）的鼠源性单克隆抗体（BC 1、BC 2和BC 3）。免疫过氧化物酶染色发现，乳腺癌组织中抗原决定簇占优势。此外，抗体优先与来自母乳的粘蛋白反应，而不是来自乳腺癌细胞系ZR 75的粘蛋白;它们还识别血清和人乳脂肪球膜中的多态性高分子量组分（MW ≥ 230 000）。因此，抗体似乎与乳腺癌和人乳中发现的粘蛋白家族的组分反应，并且似乎每个表位的至少一部分本质上是蛋白质。抗体BC 1、BC 2和BC 3识别相关但不相同的表位，并且它们似乎在与3E 1·2定义的抗原（乳腺血清抗原，MSA）相同的分子上共表达，3E 1·2定义的抗原也是乳腺癌相关粘蛋白家族的成员。然而，3E 12表位与针对BC 1、BC 2和BC 3所述的那些表位不同且不交叉反应。检查BC 2和BC 3限定的表位在血清测定中的价值。用两种抗体的组合开发了免疫测定，使用抗体BC 3用于抗原捕获，抗体BC 2或3E 1·2用于抗原检测，并且在乳腺癌的此类血清试验中给出了合理的灵敏度（~85%）和特异性（~95%）。在一项有限的研究中，这些测试似乎补充了MSA测试在乳腺癌的检测。
Three murine monoclonal antibodies (BC 1, BC2 and BC3) were developed against human milk fat globule membrane (HMFGM). By immunoperoxidase staining, it was found that the antigenic determinants had a predominant distribution in breast cancer tissue. In addition, the antibodies reacted preferentially with mucin derived from human milk rather than that derived from the breast cancer cell line ZR75; they also recognized polymorphic high molecular weight components (MW ≥ 230 000) in serum and in human milk fat globule membrane. Thus the antibodies appear to react with a component of the family of mucins found in breast cancer and human milk and it appears likely that at least part of each epitope is protein in nature. Antibodies BC1, BC2 and BC3 recognized related but not identical epitopes, and they appear to be co‐expressed on the same molecules as 3E1·2‐defined antigen (mammary serum antigen, MSA) which is also a member of the family of breast cancer‐related mucin. However, the 3E1 2 epitope is distinct and non‐cross‐reactive with those described for BC1, BC2 and BC3. The BC2 and BC3 defined epitopes were examined for their value in serum assays. Immunoassay was developed with a combination of two antibodies, using antibody BC3 for antigen capture and antibody BC2 or 3E1·2 for antigen detection and gave reasonable sensitivity (~85%) and specificity (~95%) in such serum tests for breast cancer. In a limited study, these tests appeared to complement the MSA test in the detection of breast cancer.
使用单克隆抗体 F36/22 免疫亲和分离导管癌抗原。
影响因子：
3.6
作者：
Papsidero,LD;Croghan,GA;Johnson,EA;Chu,TM
通讯作者：
Chu,TM