Enhanced functions of vascular cells on nanostructured Ti for improved stent applications
Enhanced functions of vascular cells on nanostructured Ti for improved stent applications
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DOI：
10.1089/ten.2006.0376
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发表时间：
2007-07-01
影响因子：
--
通讯作者：
Webster, Thomas J.
中科院分区：
文献类型：
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作者：
Choudhary, Saba;Haberstroh, Karen M.;Webster, Thomas J.
血管组织具有许多纳米结构的表面特征，但大多数用于恢复血液流动的金属血管支架在纳米尺度上是光滑的。因此，本研究的目的是确定纳米结构钛(Ti)与传统商业纯钛(C.P.)的体外血管细胞功能。天哪。这项研究的结果首次显示，与传统钛相比，纳米结构钛表面4小时后内皮细胞与血管平滑肌细胞的竞争性粘附性更强。此外，当单独培养时，与常规钛相比，纳米结构钛上的内皮细胞和血管平滑肌细胞密度增加。术后1、3、5d，内皮细胞在纳米结构钛表面形成融合单层，血管平滑肌细胞先于血管平滑肌细胞在纳米结构钛表面形成融合单层。结果还显示，当血管细胞在纳米结构钛上培养时，胶原和弹性蛋白的合成总量更大。由于传统血管支架失败的主要模式是与内皮细胞相比，血管内皮细胞过度生长，这些结果表明，尽管这两种类型的血管细胞的功能在纳米结构的C.P.血管内皮细胞功能(尤其重要的是细胞密度或融合)比血管平滑肌细胞增强。因此，目前的体外研究表明，血管支架由纳米C.P.组成。钛颗粒可激发有利的细胞反应，以改善支架应用。
Vascular tissue possesses numerous nanostructured surface features, but most metallic vascular stents proposed to restore blood flow are smooth at the nanoscale. Thus, the objective of the present study was to determine in vitro vascular cell functions on nanostructured titanium (Ti) compared to conventional commercially pure (c.p.) Ti. Results of this study showed for the first time greater competitive adhesion of endothelial versus vascular smooth muscle cells on nanostructured Ti compared to conventional Ti after 4 hours. Moreover, when cultured separately, increased endothelial and vascular smooth muscle cell density was observed on nanostructured Ti compared to conventionalp. Ti after 1, 3, and 5 days; endothelial cells formed confluent monolayers before vascular smooth muscle cells on nanostructured Ti. Results also showed greater total amounts of collagen and elastin synthesis by vascular cells when cultured on nanostructured Ti. Since a major mode of failure of conventional vascular stents is the overgrowth of smooth muscle cells compared to endothelial cells, these results suggest that while the functions of both types of vascular cells were promoted on nanostructured c.p. Ti, endothelial cell functions (of particular importance, cell density or confluence) were enhanced over that of vascular smooth muscle cells. Thus, the present in vitro study showed that vascular stents composed of nanometer c.p. Ti particles may invoke advantageous cellular responses for improved stent applications.