White spot syndrome virus (WSSV) in cultured Penaeus monodon in the Philippines.
White spot syndrome virus (WSSV) in cultured Penaeus monodon in the Philippines.
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菲律宾养殖斑节对虾中的白斑综合症病毒（WSSV）。
DOI：
10.3354/dao042077
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发表时间：
2000
影响因子：
1.4
通讯作者：
Lourdes Mahilum
中科院分区：
文献类型：
--
作者：
F. O. Magbanua;K. T. Natividad;V. Migo;C. Alfafara;Florian O De La Peña;Rolando O Miranda;Juan D Albaladejo;E. Cesar;B. Nadala Jr;Philip C. Loh;Lourdes Mahilum
1999年1月至5月，采用PCR（聚合酶链反应）方法和Western印迹法测定了菲律宾养殖对虾中白色斑点综合征病毒（WSSV）的感染率和地理分布。总共对71个样本进行了WSSV筛查，其中包括18个后期幼虫（PL）和53个幼虾/成虾样本（培养56至150天，DOC）。在检测的71份样品中，51份（72%）通过PCR检测到WSSV阳性：一步PCR后为61%（31/51），两步非巢式PCR后为39%（20/51）。在PL和幼虾/成年虾样品中，分别有50%和79%对WSSV呈阳性。通过Western blot检测，51份PCR阳性样品中只有6份（12%）WSSV检测阳性。20份PCR检测WSSV阴性的样品中，Western blot检测均为阴性。这是菲律宾首次报道WSSV的发生。
The prevalence and geographic distribution of white spot syndrome virus (WSSV) infection among cultured penaeid shrimp in the Philippines was determined from January to May, 1999, using PCR (polymerase chain reaction) protocol and Western blot assays. A total of 71 samples consisting of 18 post-larvae (PL) and 53 juvenile/adult shrimp samples (56 to 150 days-of-culture, DOC) were screened for WSSV. Of the 71 samples tested, 51 (72%) were found positive for WSSV by PCR: 61% (31/51) after 1-step PCR and 39% (20/51) after 2-step, non-nested PCR. Of the PL and juvenile/adult shrimp samples tested, 50 and 79% were positive for WSSV, respectively. By Western blot, only 6 of the 51 (12%) PCR-positive samples tested positive for WSSV. Of the 20 samples negative for WSSV by PCR, all tested negative for WSSV by Western blot assay. This is the first report of the occurrence of WSSV in the Philippines.