The "SUN" family:: UTH1, an ageing gene, is also involved in the regulation of mitochondria biogenesis in Saccharomyces cerevisiae
The "SUN" family:: UTH1, an ageing gene, is also involved in the regulation of mitochondria biogenesis in Saccharomyces cerevisiae
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DOI：
10.1006/abbi.1999.1655
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发表时间：
2000-03-01
影响因子：
3.9
通讯作者：
Guérin, MG
中科院分区：
文献类型：
--
作者：
Camougrand, NM;Mouassite, M;Guérin, MG
由于在先前的工作中显示NCA 3（SUN家族的四个基因之一）参与线粒体蛋白质合成调节，因此测试了该基因家族的其他成员的作用。UTH 1（而不是SUN 4或SIM 1）也被证明干扰线粒体生物合成。在Delta uth 1细胞中，细胞色素aa（3）、c和B分别降低了25%和15%。在双无效突变体Delta uth 1 Delta nca 3中，只有细胞色素aa（3）相对于野生型降低了50%。然而，在双无效突变体中，细胞呼吸与细胞色素氧化酶的比率大大提高。对整个裂解细胞的测量表明，另一种线粒体酶，柠檬酸合酶，也降低了Δ uth 1和Δ uth 1 Δ nca 3，而己糖激酶没有。电子显微照片显示，在Delta uth 1 Delta nca 3中，整体线粒体含量没有差异，但与野生型相比，线粒体对电子的密度较低。亲本和突变株的心磷脂和线粒体DNA相当。对分离的线粒体的测量表明，在Delta uth 1 Delta nca 3中，cyt aa（3）/cyt B比率也降低，但氧化酶对呼吸流量的控制更高。与野生型相比，其他线粒体复合物对氧化酶的活性在突变体中是等同的。这些结果表明，蛋白设备可以降低线粒体从UTH 1灭活的菌株。(C)北京大学出版社.
Since it was shown in previous work that NCA3 (one of the four genes of the SUN family) is involved in mitochondrial protein synthesis regulation, the effect of the other members-of this gene family was tested. UTH1 (but not SUN4 or SIM1) was also shown to interfere with mitochondria biogenesis. In Delta uth1 cells, cytochromes aa(3), c, and b were lowered by 25 and 15%, respectively. In the double-null mutant Delta uth1 Delta nca3, only cytochrome aa(3) was lowered by 50% relative to the wild type. However, the ratio of cellular respiration to cytochrome oxidase was greatly enhanced in the double-null mutant. Measurements on whole lysed cells showed that another mitochondrial enzyme, citrate synthase, was also lowered in Delta uth1 and Delta uth1 Delta nca3 whereas hexokinase was not. Electron micrographs showed no difference in global mitochondria content in Delta uth1 Delta nca3, but mitochondria appeared less dense to electrons compared to the wild type. Cardiolipin and mtDNA were equivalent in parental and mutant strains. Measurements an isolated mitochondria showed that the cyt aa(3)/cyt b ratio was also lowered in Delta uth1 Delta nca3, but the control exerted by the oxidase on the respiratory flux was higher. The activity of other mitochondrial complexes versus oxidase was equivalent in mutants compared to the wild type. These results suggest that the protein equipment could be lowered in mitochondria from strains inactivated for UTH1. (C) 2000 Academic Press.