Association of increased levels of MCP-1 and cathepsin-D in young onset type 2 diabetes patients (T2DM-Y) with severity of diabetic retinopathy
Association of increased levels of MCP-1 and cathepsin-D in young onset type 2 diabetes patients (T2DM-Y) with severity of diabetic retinopathy
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DOI：
10.1016/j.jdiacomp.2017.02.017
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发表时间：
2017-05-01
影响因子：
3
通讯作者：
Balasubramanyam, Muthuswamy
中科院分区：
文献类型：
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作者：
Reddy, Sruthi;Amutha, Anandakumar;Balasubramanyam, Muthuswamy
目的：年轻发病的2型糖尿病患者（T2DM-Y）发生微血管并发症的风险增加，尤其是糖尿病视网膜病变。然而，分子机制尚不清楚。在这项研究中，我们研究了T2DM-Y患者血清单核细胞趋化蛋白1 （MCP-1）和组织蛋白酶- d的水平。方法：在本病例对照研究中，参与者包括糖耐量正常（NGT = 40）、2型糖尿病（T2DM = 35）、非增殖性糖尿病视网膜病变（NPDR = 35）和增殖性糖尿病视网膜病变（PDR = 35）患者。研究对象的临床特征按标准程序完成，MCP-1和组织蛋白酶- d用ELISA法测定。结果：与对照组相比，T2DM-Y、NPDR和PDR患者的MCP-1水平显著升高（p < 0.001）。T2DM-Y患者的Cathepsin-D水平也显著升高（p < 0.001）。相关分析显示MCP-1与组织蛋白酶- d水平呈正相关（p < 0.001）。MCP1/cathepsin-D也与c肽水平呈显著负相关。即使在调整了所有混杂因素后，DR患者中MCP-1/cathepsin-D水平升高的相关性仍然存在。结论：MCP-1和组织蛋白酶- d都是细胞衰老的分子标志，我们认为这些生物标志物可能有助于预测T2DM-Y患者视网膜病变的发展。(C) 2017爱思唯尔公司版权所有。
Aim: Young onset type 2 diabetes patients (T2DM-Y) have been shown to possess an increased risk of developing microvascular complications particularly diabetic retinopathy. However, the molecular mechanisms are not clearly understood. In this study, we investigated the serum levels of monocyte chemotactic protein 1 (MCP-1) and cathepsin-D in patients with T2DM-Y without and with diabetic retinopathy.Methods: In this case-control study, participants comprised individuals with normal glucose tolerance (NGT = 40), patients with type 2 diabetes mellitus (T2DM = 35), non-proliferative diabetic retinopathy (NPDR = 35) and proliferative diabetic retinopathy (PDR = 35). Clinical characterization of the study subjects was done by standard procedures and MCP-1 and cathepsin-D were measured by ELISA.Results: Compared to control individuals, patients with T2DM-Y, NPDR and PDR exhibited significantly (p < 0.001) higher levels of MCP-1. Cathepsin-D levels were also significantly (p < 0.001) higher in patients with T2DM-Y without and with diabetic retinopathy. Correlation analysis revealed a positive association (p < 0.001) between MCP-1 and cathepsin-D levels. There was also a significant negative correlation of MCP1/cathepsin-D with C-peptide levels. The association of increased levels of MCP-1/cathepsin-D in patients with DR persisted even after adjusting for all the confounding factors.Conclusion: As both MCP-1 and cathepsin-D are molecular signatures of cellular senescence, we suggest that these biomarkers might be useful to predict the development of retinopathy in T2DM-Y patients. (C) 2017 Elsevier Inc. All rights reserved.