A factor binding GATAAG confers tissue specificity on the promoter of the human zeta-globin gene.
A factor binding GATAAG confers tissue specificity on the promoter of the human zeta-globin gene.
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结合因子 GATAAG 赋予人类 zeta 珠蛋白基因启动子组织特异性。
DOI：
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发表时间：
1990
影响因子：
14.9
通讯作者：
N. Proudfoot
中科院分区：
文献类型：
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作者：
P. Watt;P. Lamb;L. Squire;N. Proudfoot
我们描述了控制人类zeta珠蛋白基因组织特异性表达的顺式作用序列的特征。对该基因附近增强子序列的广泛搜索证明是阴性的。相反，我们的数据表明zeta基因的最小启动子本身是组织特异性的。接近并可能包括-100 CACCC和-70 CCAAT盒子的序列显示出一定的红系特异性。然而，主要的组织特异性元件是与最小启动子直接相邻的-120的GATAA序列。GATAA的特异性缺失使红系细胞的zeta启动子活性降低了5倍，而非红系细胞则没有。我们还证明了一个红系特异性因子与这个GATAA序列结合。此外，该因子与转录因子CP1形成复合物，我们显示其与zeta CCAAT盒相互作用。我们提供的证据表明，zeta GATAA结合因子相当于最近由Tsai等人纯化和克隆的GF1。在许多红系特异性基因的启动子和增强子中发现了红系特异性GATAA序列。同样，我们在这里表明，zeta珠蛋白基因依赖于其启动子中的GATAA序列来指定其在红细胞中的表达。
We describe the characterisation of cis-acting sequences which control the tissue specific expression of the human zeta globin gene. An extensive search for enhancer sequences in the vicinity of this gene proved negative. Instead our data demonstrate that the minimal promoter of the zeta gene is itself tissue specific. Sequences close to and possibly including the -100 CACCC and -70 CCAAT boxes display some erythroid specificity. However the principal tissue specific element is a GATAA sequence at -120 directly adjacent to the minimal promoter. Specific deletion of GATAA reduces zeta promoter activity 5 fold in erythroid but not non-erythroid cells. We also demonstrate that an erythroid specific factor binds to this GATAA sequence. Furthermore this factor forms a complex with the transcription factor CP1 which we show interacts with the zeta CCAAT box. We present evidence that the zeta GATAA binding factor is equivalent to GF1 recently purified and cloned by Tsai et al [1]. The erythroid specific GATAA sequence has been found in the promoters and enhancers of a number of erythroid specific genes. Similarly we show here that the zeta globin gene relies on a GATAA sequence in its promoter to specify its expression in erythroid cells.