Developmentally regulated gene expression of all eight metabotropic glutamate receptors in hypothalamic suprachiasmatic and arcuate nuclei - a PCR analysis
Developmentally regulated gene expression of all eight metabotropic glutamate receptors in hypothalamic suprachiasmatic and arcuate nuclei - a PCR analysis
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DOI：
10.1016/s0165-3806(97)00066-7
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发表时间：
1997-08-18
期刊：
影响因子：
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通讯作者：
vandenPol, AN
中科院分区：
文献类型：
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作者：
Ghosh, PK;Baskaran, N;vandenPol, AN
先前的研究已经证明了谷氨酸在下丘脑中的关键作用，无论是在发育中还是在成年大脑中。研究了视交叉上核（SCN）和弓状核（ARC）中代谢型谷氨酸受体（mGluR）mRNA（mGluR 1 -8）的表达。通过反向北方杂交和cDNA-PCR，我们发现所有8个克隆的mGluRs在这些脑区表达。大多数以前在这里没有被发现。令人惊讶的是，这包括以前被认为仅限于视网膜的mGluR，如mGluR 6。我们还检测，克隆和测序的mGluR 7（mGluR 7 b）的剪接变体。在发育上，mGluRs最大表达的年龄依赖于该区域。例如，mGluR 5在新生儿ARC中比在成人中更强地表达，而在SCN中则相反。与P10新生儿相比，mGluR 1、R3、R6、R7 a、R7 b和R8在成人SCN和ARC中的表达更高。(C)1997年Elsevier Science B.V.
Previous studies have demonstrated the critical role glutamate plays in the hypothalamus, both in the developing and adult brain. The expression of metabotropic glutamate receptor (mGluR) mRNA (mGluR1-8) was studied in the suprachiasmatic (SCN) and arcuate (ARC) nuclei. Using reverse Northern blots and cDNA-PCR, we found that all eight cloned mGluRs were expressed in these brain regions. Most had not previously been detected here. Surprisingly, this included mGluRs that had previously been thought to be restricted to the retina, such as mGluR6. We also detected, cloned, and sequenced a splice variant of mGluR7 (mGluR7b). Developmentally, the age of maximal expression of mGluRs was dependent on the region. For instance, mGluR5 was more strongly expressed in neonatal ARC than in adult, whereas the opposite was true in the SCN. Compared with P10 neonates, mGluR1, R3, R6, R7a, R7b, and R8 showed a greater expression in adult SCN and ARC. (C) 1997 Elsevier Science B.V.