Reduction in levels of amphiphysin 1 mRNA in the hippocampus of aged rats subjected to repeated variable stress
Reduction in levels of amphiphysin 1 mRNA in the hippocampus of aged rats subjected to repeated variable stress
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DOI：
10.1016/j.neuroscience.2004.03.001
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发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Nishida, A
中科院分区：
文献类型：
--
作者：
Sawada, T;Morinobu, S;Nishida, A
关于衰老的各种神经生物学研究表明，循环糖皮质激素水平升高导致海马对应激的脆弱性，尽管对老年人应激脆弱性的分子机制知之甚少。我们比较了老年（20个月）和成年（30个月）大鼠海马中的基因表达谱，重复可变应力（RVS）4天，使用cDNA阵列技术和实时定量PCR，以确定参与老年人应激脆弱性机制的推定基因。我们发现一个显着的水平下降amphiphysin 1的mRNA在老年大鼠进行RVS相比，治疗和未治疗的成年大鼠或未治疗的老年大鼠。类似地，我们发现在经历8天RVS的老年大鼠中海马am phiphysin 1 mRNA水平显著降低，但在经历单次VS的那些中没有。这些发现表明海马am phiphysin 1 mRNA水平响应于重复应激的降低可能涉及老年人的应激脆弱性，并可能导致老年人在应激条件下的学习和记忆障碍。(C)2004年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Various neurobiological studies of aging indication that elevated levels of circulating glucocorticoids lead to hippocampal vulnerability to stress, though little is knowing about the molecular mechanism underlying stress vulnerability in the elderly. We have compared the gene expression profiles in the hippocampus of aged (20 months) and adult (30 months) rats in response to repeated variable stress (RVS) for 4 days, using a cDNA array technique and real-time quantitative PCR, to identify putative genes involved in the mechanism of stress vulnerability in the elderly. We found a significant decrease in the levels of amphiphysin 1 mRNA in aged rats subjected to RVS compared with treated and un treated adult rats or to untreated aged rats. Similarly, we found a significant decrease in hippocampal levels of am phiphysin 1 mRNA in aged rats subjected to RVS for 8 days but not in those subjected to a single VS. These findings suggest that the decrease in the hippocampal levels of am phiphysin 1 mRNA in response to repeated stress may be involved in the stress vulnerability in the elderly, and may lead to the disturbance of learning and memory under stressful conditions in the elderly. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.