Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet.
Dietary sodium chloride attenuates increased β-cell mass to cause glucose intolerance in mice under a high-fat diet.
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饮食氯化钠减弱增加的β-细胞质量，导致高脂饮食小鼠的葡萄糖耐受不良。
DOI：
10.1371/journal.pone.0248065
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Arima H
中科院分区：
文献类型：
--
作者：
Taki K;Takagi H;Hirose T;Sun R;Yaginuma H;Mizoguchi A;Kobayashi T;Sugiyama M;Tsunekawa T;Onoue T;Hagiwara D;Ito Y;Iwama S;Suga H;Banno R;Sakano D;Kume S;Arima H
过量的钠盐（NaCl）或脂肪摄入与各种增加的健康风险有关。然而，是否过量的NaCl摄入伴随着高脂肪饮食（HFD）影响葡萄糖代谢尚未阐明。在这项研究中，C57 BL/6 J雄性小鼠被喂食正常食物饮食（NCD）、NCD+高NaCl饮食（NCD + NaCl）、HFD或HFD+高NaCl饮食（HFD + NaCl）30周。在NCD喂养的小鼠和NCD加NaCl喂养的小鼠之间没有观察到体重增加、胰岛素敏感性和葡萄糖耐量的显著差异。相比之下，与喂食HFD的小鼠相比，喂食HFD + NaCl的小鼠的体重和肝脏重量降低，但附睾白色脂肪组织的重量增加。与HFD喂养的小鼠相比，HFD加NaCl喂养的小鼠在胰岛素耐量试验中具有较低的血浆葡萄糖水平，并且在腹膜内葡萄糖耐量试验中显示出较高的血浆葡萄糖和较低的血浆胰岛素水平。与喂食HFD的小鼠相比，喂食HFD加氯化钠的小鼠的β细胞面积和胰岛数量减少。与NCD喂养的小鼠相比，在HFD喂养的小鼠中观察到Ki 67阳性β细胞增加，以及胰岛中Ki 67、CyclinB 1和CyclinD 1 mRNA表达水平增加，但在HFD + NaCl喂养的小鼠中未观察到。我们的数据表明，过量的NaCl摄入伴随HFD会加重葡萄糖耐受不良，胰腺中β细胞团扩张的减弱会导致胰岛素分泌受损。
Excessive sodium salt (NaCl) or fat intake is associated with a variety of increased health risks. However, whether excessive NaCl intake accompanied by a high-fat diet (HFD) affects glucose metabolism has not been elucidated. In this study, C57BL/6J male mice were fed a normal chow diet (NCD), a NCD plus high-NaCl diet (NCD plus NaCl), a HFD, or a HFD plus high-NaCl diet (HFD plus NaCl) for 30 weeks. No significant differences in body weight gain, insulin sensitivity, and glucose tolerance were observed between NCD-fed and NCD plus NaCl-fed mice. In contrast, body and liver weights were decreased, but the weight of epididymal white adipose tissue was increased in HFD plus NaCl-fed compared to HFD-fed mice. HFD plus NaCl-fed mice had lower plasma glucose levels in an insulin tolerance test, and showed higher plasma glucose and lower plasma insulin levels in an intraperitoneal glucose tolerance test compared to HFD-fed mice. The β-cell area and number of islets were decreased in HFD plus NaCl-fed compared to HFD-fed mice. Increased Ki67-positive β-cells, and increased expression levels of Ki67, CyclinB1, and CyclinD1 mRNA in islets were observed in HFD-fed but not HFD plus NaCl-fed mice when compared to NCD-fed mice. Our data suggest that excessive NaCl intake accompanied by a HFD exacerbates glucose intolerance, with impairment in insulin secretion caused by the attenuation of expansion of β-cell mass in the pancreas.
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DOI：
10.1073/pnas.0608703104
发表时间：
2007-05-22
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
6.9
作者：
Fonseca-Alaniz, Miriam H.;Takada, Julie;Lima, Fabio B.
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影响因子：
9.8
作者：
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