The pathogenesis of gonococcal urethritis in men: Confocal and immunoelectron microscopic analysis of urethral exudates from men infected with Neisseria gonorrhoeae
The pathogenesis of gonococcal urethritis in men: Confocal and immunoelectron microscopic analysis of urethral exudates from men infected with Neisseria gonorrhoeae
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DOI：
10.1093/infdis/173.3.636
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发表时间：
1996-03-01
影响因子：
6.4
通讯作者：
Blake, MS
中科院分区：
文献类型：
--
作者：
Apicella, MA;Ketterer, M;Blake, MS
对12例男性淋球菌性尿道炎患者尿道分泌物的共聚焦和免疫电镜分析表明，淋球菌可侵入尿道上皮细胞，吖啶橙子染色研究表明，尿道上皮细胞内的大多数微生物在固定时是活的，使用YOYO-1和DiIC染色的21个数字化共聚焦切片的图像分析对感染的上皮细胞进行三维建模（18）（3）显示淋球菌以极性方式侵入这些细胞，最有可能在上皮腔表面。系列免疫电镜照片显示淋球菌和上皮细胞之间的膜融合和基座形成的证据，空泡内的淋球菌，和偶尔的胞质中的游离淋球菌，免疫电镜研究显示细胞表面空泡破裂，释放生物体。这些研究表明，在男性感染过程中，尿道上皮细胞被淋球菌侵入。
Confocal and immunoelectron microscopic analysis of urethral exudates from 12 men with gonococcal urethritis showed that Neisseria gonorrhoeae can invade urethral epithelial cells, Studies with acridine orange stain demonstrated that the majority of organisms within urethral epithelial cells were viable at the time of fixation, Three-dimensional modeling of an infected epithelial cell using image analysis of 21 digitized confocal sections stained with YOYO-1 and DiIC(18)(3) revealed that gonococcal invasion of these cells occurred in a polar fashion, most likely at the epithelial luminal surface, Serial immunoelectron micrographs showed evidence of membrane fusion with pedestal formation between the gonococcus and the epithelial cell, gonococci within vacuoles, and occasional gonococci free in the cytoplasm, Immunoelectron microscopy studies showed ruptured vacuoles at the cell surface releasing organisms, These studies demonstrate that urethral epithelial cells are invaded by gonococci during the course of infection in males.