A novel RNA sequencing-based miRNA signature predicts with recurrence and outcome of hepatocellular carcinoma
A novel RNA sequencing-based miRNA signature predicts with recurrence and outcome of hepatocellular carcinoma
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一种基于 RNA 测序的新型 miRNA 特征可预测肝细胞癌的复发和结果
DOI：
10.1002/1878-0261.12315
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发表时间：
2018-06-01
影响因子：
6.6
通讯作者：
Meng, Qing H.
中科院分区：
文献类型：
--
作者：
Bai, Fumao;Zhou, Huaibin;Meng, Qing H.
肝细胞癌 (HCC) 是全球第五大最常见的癌症类型，也是全球癌症相关死亡的第二大原因。鉴于肝切除术后5年HCC复发率高达70%，HCC患者通常预后较差。可以预测疾病复发的生物标志物或一组生物标志物将产生重大的临床影响，从而可以更早地检测复发并进行更有效的治疗。为了确定与 HCC 复发相关的新 microRNA (miRNA) 特征，我们分析了 306 名 HCC 患者的数据，这些患者的 miRNA 表达谱和完整的临床信息均来自癌症基因组图谱数据库。通过这项分析，我们确定了一个能够有效预测患者复发风险的 6-miRNA 特征；高风险组和低风险组的无复发生存率存在显着差异。与时间相关的接收器操作特性分析表明该特征具有良好的预测性能。多变量 Cox 回归和分层分析表明，六种 miRNA 特征独立于其他临床特征。对六种预后 miRNA 的基因靶标进行功能富集分析表明，富集主要集中在癌症相关通路和重要的细胞生物学过程中。我们的结果支持使用这六种 miRNA 特征作为预测 HCC 患者复发和结果的独立因素。
Hepatocellular carcinoma (HCC) is the fifth most common type of cancer and the second leading cause of cancer-related deaths worldwide. Given that the rate of HCC recurrence 5 years after liver resection is as high as 70%, patient with HCC typically has a poor outcome. A biomarker or set of biomarkers that could predict disease recurrence would have a substantial clinical impact, allowing earlier detection of recurrence and more effective treatment. With the aim of identifying a new microRNA (miRNA) signature associated with HCC recurrence, we analyzed data on 306 patients with HCC for whom both miRNA expression profiles and complete clinical information were available from The Cancer Genome Atlas database. Through this analysis, we identified a six-miRNA signature that could effectively predict patients' recurrence risk; the high-risk and low-risk groups had significantly different recurrence-free survival rates. Time-dependent receiver operating characteristic analysis indicated that this signature had a good predictive performance. Multivariable Cox regression and stratified analyses demonstrated that the six-miRNA signature was independent of other clinical features. Functional enrichment analysis of the gene targets of the six prognostic miRNA indicated enrichment mainly in cancer-related pathways and important cell biological processes. Our results support use of this six-miRNA signature as an independent factor for predicting recurrence and outcome of patients with HCC.