Development of Low Cost Rapid Fabrication of Sharp Polymer Microneedles for In Vivo Glucose Biosensing Applications
Development of Low Cost Rapid Fabrication of Sharp Polymer Microneedles for In Vivo Glucose Biosensing Applications
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DOI：
10.1149/2.0141510jss
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发表时间：
2015-01-01
影响因子：
2.2
通讯作者：
O'Riordan, Alan
中科院分区：
文献类型：
--
作者：
Barrett, Colm;Dawson, Karen;O'Riordan, Alan
正在开发能够更有效地管理糖尿病的新方法，例如连续葡萄糖监测，以防止低血糖或高血糖的不稳定发作，并提供改善的用户体验。一种实现这些要求的新兴方法是制造用于直接体内检测组织液中的葡萄糖的微创经皮传感器。针头--长度小于1 mm的尖锐的显微结构--先前已被用于无痛穿透角质层以递送药物和疫苗。在这项工作中，我们提出，超尖锐的金涂层聚合物微针阵列（亚微米尖端半径），这是使用低成本的聚合物复制方法制造。微针阵列的关键尺寸的特征在于使用光学和扫描电子显微镜的组合。制造的微针装置通过循环伏安法表征以探索功能性。在葡萄糖氧化酶存在下，以二茂铁一元羧酸为氧化介质，进行葡萄糖的伏安检测。该生物传感器可应用于生理范围（2 - 13.5 mM）内葡萄糖的定量。该传感器对葡萄糖具有高选择性，其他可氧化物质（包括尿酸、抗坏血酸、甘露糖、果糖、水杨酸（阿司匹林）和对乙酰氨基酚（扑热息痛））的干扰可忽略不计。这证明了这些微针装置在体内葡萄糖检测中的潜在未来用途。(C)作者（S）2015由ECS发布。这是一篇开放获取的文章，根据Creative Commons Attribution Non-Commercial No Derivatives 4.0 License（CC BY-NC-ND，http：//creativecommons.org/licenses/by-nc-nd/4.0/）的条款进行分发，该许可证允许在任何媒体上进行非商业性的重复使用、分发和复制，前提是原始作品未以任何方式进行更改，并且引用正确。如需商业再利用许可，请发送电子邮件至oa@electrochem.org。保留所有权利。
New approaches to enable more effective management of diabetes mellitus, such as continuous glucose monitoring are being developed both to prevent unstable episodes of hypo or hyper glycaemia and also to provide an improved user experience. One emerging approach to realize these requirements is to fabricate a minimally invasive transdermal sensor for the direct in vivo detection of glucose in the interstitial fluid. Microneedles - sharp, microscopic structures measuring less than 1 mm in length - have been previously employed to allow painless penetration of the stratum corneum for delivery of drugs and vaccines. In this work we present, ultra sharp gold coated polymer microneedle arrays (sub micron tip radii) which are fabricated using a low cost polymer replication approach. Critical dimensions of the microneedle arrays are characterized using a combination of optical and scanning electron microscopies. Fabricated microneedle devices are characterized by cyclic voltammetry to explore functionality. The voltammetric detection of glucose is performed using ferrocene monocarboxylic acid as an oxidising mediator in the presence of glucose oxidase. The biosensor can be applied to the quantification of glucose in the physiological range (2 - 13.5 mM). The sensors demonstrate high selectivity towards glucose with negligible interference from other oxidizable species including uric acid, ascorbic acid, mannose, fructose, salicylic acid (Aspirin) and acetaminophen (Paracetamol). This demonstrates potential future use of these microneedle devices for in vivo glucose detection.(C) The Author(s) 2015. Published by ECS. This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives 4.0 License (CC BY-NC-ND, http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is not changed in any way and is properly cited. For permission for commercial reuse, please email: oa@electrochem.org. All rights reserved.