Fabrication of amphiphilic copolymeric gels with enhanced activity of immobilized enzymes in organic media
Fabrication of amphiphilic copolymeric gels with enhanced activity of immobilized enzymes in organic media
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DOI：
10.1002/app.41905
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发表时间：
2015-05-05
影响因子：
3
通讯作者：
Tokuyama, Hideaki
中科院分区：
文献类型：
--
作者：
Sato, Ryuichi;Sato, Ayumi;Tokuyama, Hideaki
研制了一种新型的两亲性共聚凝胶用于脂肪酶的固定化。以N-异丙基丙烯酰胺（NIPA）为温敏性两亲组分，聚乙二醇甲醚丙烯酸酯（PEGMEA）为亲水组分，在水溶液中进行共聚反应，制备了NIPA-co-PEGMEA凝胶。由于聚（NIPA）的热敏性和两亲性，该凝胶可以在高于下临界溶液温度的温度下吸收有机溶剂。固定在NIPA：PEGMEA组成为950：50 mol/m（3）的NIPA-共-PEGMEA凝胶内的脂肪酶成功地催化油酸和乙醇的酯化，在20-40 ℃的重复使用期间没有活性损失。固定化脂肪酶的活力明显高于游离脂肪酶。NIPA-co-PEGMEA凝胶提供了一种结构，该结构允许固定化的脂肪酶在水性环境中以脂肪酶在凝胶中的分散状态活跃地工作。(c)2015 Wiley Periodicals，Inc. J.应用聚合物Sci. 2015，132，41905。
Novel amphiphilic copolymeric gels were developed to immobilize lipase. NIPA-co-PEGMEA gels were prepared by copolymerizing N-isopropylacrylamide (NIPA) as a thermosensitive and amphiphilic component and poly(ethylene glycol) methyl ether acrylate (PEGMEA) as a hydrophilic component in aqueous media. The gels can absorb organic solvents at temperatures higher than the lower critical solution temperature owing to the thermosensitive and amphiphilic properties of poly(NIPA). The lipase immobilized within the NIPA-co-PEGMEA gel, which had a NIPA : PEGMEA composition of 950 : 50 mol/m(3), successfully catalyzed the esterification of oleic acid and ethanol without loss of activity during repeated use within 20-40 degrees C. The activity of the immobilized lipase was considerably higher than that of free lipase. The NIPA-co-PEGMEA gels provide a structure that allows the immobilized lipase to work actively in an aqueous environment and with the dispersed state of the lipase in the gels. (c) 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41905.