SERUM-ALBUMIN BINDING OF PALMITATE AND STEARATE - MULTIPLE BINDING THEORY FOR INSOLUBLE LIGANDS
SERUM-ALBUMIN BINDING OF PALMITATE AND STEARATE - MULTIPLE BINDING THEORY FOR INSOLUBLE LIGANDS
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DOI：
10.1111/j.1432-1033.1989.tb14795.x
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发表时间：
1989-06-01
期刊：
影响因子：
--
通讯作者：
PEDERSEN, AO
中科院分区：
文献类型：
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作者：
BRODERSEN, R;VORUM, H;PEDERSEN, AO
在配体与载体结合的通常研究中，在具有不同载体和配体浓度的平衡混合物中测量自由和结合的配体浓度。然后用诸如Scatchard’s之类的结合方程或一般结合方程来分析观测到的数据。用棕榈酸、硬脂酸和油酸作为配体，我们发现水溶解度太低，不允许进行这一过程。因此，我们对一般的结合方程进行了变换，使它不包含与配体水溶液有关的参数。经典的结合方程描述了配体从水溶液到载体转移的亲和性，而新方程适用于配体从一种结合状态转移到另一种结合状态，即相对结合描述。相对绑定常数，L1, L2, L3…因此，在新方程中，Li定义了配体从与标准载体的1:1配合物到与所研究载体的i:1配合物的转移亲和力。通过测定相同脂肪酸/白蛋白溶液的透析交换速率，研究了棕榈酸酯和硬脂酸酯与人血清白蛋白的结合。在不考虑水溶液中配体的情况下，用新方程对结果进行了分析。
In usual stidues of ligand binding to a carrier, free and bound ligand concentrations are measured in equilibrium mixtures with varying carrier and ligand concentrations. The observed data are then analyzed by a binding equation such as Scatchard''s or the general binding equation. With palmitic, stearic and oleic acids as ligands we found that the aqueous solubility is too low to allow this procedure. We have consequently transformed the general binding equation so that it does not contain parameters related to aqueous solutions of the ligand. While the classical binding equations describe affinities of transfer of a ligand from an aqueous solution to the carrier, the new equation is valid for transfer of a ligand from one bound state to another, i.e. for relative binding description. The relative binding constants, L1, L2, L3 ...Li, in the new equation thus define the transfer affinity for the ligand from a 1:1 complex witha standard carrier to an i:1 complex of the ligand with the carrier investigated. Binding of palmitate and stearate to human serum albumin was studied by determination of dialytic exchange rates between identical fatty acid/albumin solutions. The results were analyzed by the new equation without reference to ligands in aqueous solution.