Crystalline alcohol dehydrogenase from baker's yeast.
Crystalline alcohol dehydrogenase from baker's yeast.
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来自面包酵母的结晶醇脱氢酶。
DOI：
10.1016/s0021-9258(19)51151-6
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发表时间：
1950
期刊：
影响因子：
--
通讯作者：
E. Racker
中科院分区：
文献类型：
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作者：
E. Racker
Negelein和Wulff（1）从啤酒酵母中纯化并结晶了乙醇脱氢酶，该酶催化乙醇可逆氧化为乙醛。这些作者所描述的过程是费力和耗时的，特别是用美国啤酒酵母，产量很小。本文的目的是报道用一种简单的方法从面包酵母中分离和结晶醇脱氢酶，其产率约为浸渍汁中酶活性的50%。在1个工作日内可从浸渍汁中获得结晶酶。另外两个实验室也重复了这一过程，取得了类似的成功。该酶是研究歧化反应和测定二磷酸吡啶核苷酸（DPN）的有用工具，也被用作DPN连接酶反应动力学研究的模型。
Alcohol dehydrogenase, the enzyme which catalyzes the reversible oxidation of alcohol to acetaldehyde, was purified and crystallized from brewers’ yeast by Negelein and Wulff (1). The procedure described by these authors is laborious and time-consuming, and, particularly with American brewers’ yeast, the yield is small. It is the purpose of this paper to report the isolation and crystallization of alcohol dehydrogenase from bakers’ yeast by a simple procedure resulting in a yield of about 50 per cent of the enzyme activity present in the maceration juice. The crystalline enzyme can be obtained from the maceration juice within 1 working day. The procedure has been repeated in two other laboratories with similar success. l The enzyme is a useful tool for the study of dismutation reactions and for the determination of diphosphopyridine nucleotide (DPN), and has also been used as a model for kinetic studies of DPN-linked enzyme reactions.