Antimicrobial Resistance and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus Isolates from Child Patients of High-Risk Wards in Shenzhen, China.
Antimicrobial Resistance and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus Isolates from Child Patients of High-Risk Wards in Shenzhen, China.
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DOI：
10.7883/yoken.jjid.2016.328
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发表时间：
2017-02
影响因子：
2.2
通讯作者：
Yang Qin;Feiqiu Wen;Yuejie Zheng;R. Zhao;Q. Hu;Renli Zhang
中科院分区：
文献类型：
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作者：
Yang Qin;Feiqiu Wen;Yuejie Zheng;R. Zhao;Q. Hu;Renli Zhang
耐甲氧西林金黄色葡萄球菌 (MRSA) 菌株导致高死亡率，从而给全世界的公共卫生带来沉重负担。在这里，我们调查了从深圳市儿童医院儿童患者中分离出的 MRSA 的抗菌敏感性和分子特征。我们通过抗菌药物敏感性测试对 140 种 MRSA 菌株进行了鉴定。我们进一步进行了 spa 分型、多位点序列分型 (MLST)、葡萄球菌盒式染色体 mec (SCCmec) 分析、pvl 基因分析和脉冲场凝胶电泳 (PFGE)。分析发现 MRSA 菌株对大多数非 β-内酰胺抗菌剂敏感。序列类型 (ST) 59 被发现是最常见的 MLST 谱系 (54.3%)。大多数 MRSA 分离株属于 SCCmec IV (64.3%) 和 V (22.8%) 类型。 MRSA-ST59-SCCmec IV-t437 克隆是最主要的菌株，感染了所有研究患者的 28.6%。此外，50.7% 的 MRSA 分离株被发现呈 pvl 阳性。我们报告了深圳市儿童医院 MRSA 基因型流行率和分布的初步数据。我们对中国儿童患者中 MRSA 的定植动态进行了分析，我们的研究结果可以作为制定预防 MRSA 感染和传播策略的基础。
Methicillin-resistant Staphylococcus aureus (MRSA) strains are responsible for high rates of mortality and thus pose a substantial burden to public health worldwide. Here, we investigated the antimicrobial susceptibility and molecular characteristics of MRSA isolated from child patients at Shenzhen Children's Hospital. We characterized 140 MRSA strains through antimicrobial susceptibility testing. We further performed spa typing, multilocus sequence typing (MLST), staphylococcal cassette chromosome mec (SCCmec) analysis, pvl gene analysis, and pulsed-field gel electrophoresis (PFGE). The analyzed MRSA strains were found to be sensitive to most non-β-lactam antimicrobial agents. Sequence type (ST) 59 was found to be the most common MLST lineage (54.3%). Most MRSA isolates belonged to the SCCmec IV (64.3%) and V (22.8%) types. The MRSA-ST59-SCCmec IV-t437 clone was the most predominant strain that infected 28.6% of all patients studied. Moreover, 50.7% of MRSA isolates were found to be pvl-positive. We report preliminary data on the prevalence and distribution of MRSA genotypes in Shenzhen Children's Hospital. We characterized MRSA colonization dynamics in child patients in China, and our findings can serve as the basis for the development of strategies to prevent MRSA infection and transmission.