Chronic unpredictable stress impairs endogenous antioxidant defense in rat brain
Chronic unpredictable stress impairs endogenous antioxidant defense in rat brain
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DOI：
10.1016/j.neulet.2014.10.031
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发表时间：
2015-01-01
影响因子：
2.5
通讯作者：
Wang, Jun-Feng
中科院分区：
文献类型：
--
作者：
Che, Yi;Zhou, Zhu;Wang, Jun-Feng
许多研究表明，慢性应激可导致神经元损伤和抑郁，但其确切机制仍不清楚。神经元易受脂质过氧化诱导的损伤，因为神经元细胞膜的主要部分是多不饱和脂肪酸，其是活性氧的底物。由于内源性抗氧化防御系统通常会消除活性氧的产生，因此抗氧化防御不足会导致氧化应激诱导的损伤。在本研究中，了解内源性抗氧化防御在慢性应激诱导的神经元损伤的作用，我们分析了脂质过氧化，总抗氧化能力，和过氧化氢酶和谷胱甘肽过氧化物酶的活性在额叶皮层，海马和纹状体的慢性不可预测的压力暴露的大鼠。我们发现，慢性不可预测的压力，在大鼠四周诱导抑郁样行为，如快感缺乏，绝望和减少探索。慢性不可预见性应激大鼠脑组织脂质过氧化产物丙二醛含量增加，总抗氧化能力、谷胱甘肽过氧化物酶活性和过氧化氢酶活性降低。我们的研究结果表明，下调内源性抗氧化防御诱导脂质过氧化作用，慢性应激和抑郁症的作用。(C)2014爱思唯尔爱尔兰有限公司版权所有。
Many studies have shown that chronic stress can cause neuronal damage and depression, but this exact mechanism still remains unknown. Neurons are vulnerable to lipid peroxidation-induced damage because the major part of neuronal cell membrane is polyunsaturated fatty acids that are substrate for reactive oxygen species. Since endogenous antioxidant defense systems normally eliminate production of reactive oxygen species, deficient antioxidant defense can cause oxidative stress-induced damage. In the present study, to understand the role of endogenous antioxidant defense in chronic stress-induced neuronal damage, we analyzed lipid peroxidation, total antioxidant capacity, and activities of catalase and glutathione peroxidase in frontal cortex, hippocampus and striatum of rats exposed to chronic unpredictable stress. We found that chronic unpredictable stress for four weeks in rats induced depressive-like behaviors such as anhedonia, despair and decreased exploration. Malondialdehyde, a lipid peroxidation product, is increased, but total antioxidant capacity, glutathione peroxidase activity and catalase activity are decreased in brain of rats exposed to chronic unpredictable stress. Our findings suggest that down regulation of endogenous antioxidant defense induces lipid peroxidation contributing a role to chronic stress and depression. (C) 2014 Elsevier Ireland Ltd. All rights reserved.