DETERMINATION OF FIXED CHARGE-DENSITY IN CARTILAGE USING NUCLEAR-MAGNETIC-RESONANCE
DETERMINATION OF FIXED CHARGE-DENSITY IN CARTILAGE USING NUCLEAR-MAGNETIC-RESONANCE
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DOI：
10.1002/jor.1100100102
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发表时间：
1992-01-01
影响因子：
2.8
通讯作者：
BURSTEIN, D
中科院分区：
文献类型：
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作者：
LESPERANCE, LM;GRAY, ML;BURSTEIN, D
软骨的许多生物力学和化学性质依赖于细胞外基质的固定电荷密度（FCD）。 在这项研究中，核磁共振（NMR）光谱作为一种非破坏性的技术，用于确定软骨FCD。 通过NMR测量软骨外植体中的钠含量，并与通过电感耦合等离子体发射光谱（ICP）测量的钠含量进行比较，以验证软骨中钠的总NMR可见性。 NMR与ICP结果的比值为1.02 +/- 0.04（小牛，平均值+/- SD，n = 7）和1.04 +/- 0.11（成年牛，n = 8）。 通过NMR测量的钠浓度然后与理想唐南理论一起用于计算FCD的估计值。 对于接近生理条件的小牛关节软骨（AC），计算的FCD为-0.28 +/- 0.03 M（n = 10）。 然后对在不同盐组成、pH或离子强度的浴中顺序平衡的单个软骨样本进行NMR测量。 对于小牛和成年AC，计算的FCD在pH 3和2之间急剧下降，成年标本带正电荷，但小牛组织保留净负电荷。 对于在0.3-0.015 M NaCl中平衡的小牛AC，观察到计算的FCD随浴离子强度的降低而略微降低。 对于骺软骨，FCD随着关节内外植体的来源位置而变化，范围为-0.19至-0.35 M，与组织糖胺聚糖含量相关。 初步的核磁共振成像实验表明，在完整的尺骨骺软骨钠浓度的变化类似。 总的来说，这些结果证明了NMR在软骨中非破坏性地跟踪FCD的能力。 该技术适用于动态研究以及活组织的体外和体内研究。
Many biomechanical and chemical properties of cartilage are dependent on the fixed charge density (FCD) of the extracellular matrix. In this study, nuclear magnetic resonance (NMR) spectroscopy was investigated as a nondestructive technique for determining FCD in cartilage. Sodium content was measured by NMR in cartilage explants and was compared with sodium content measured by inductively coupled plasma emission spectroscopy (ICP) in order to verify the total NMR visibility of sodium in cartilage. The ratio of NMR to ICP results was 1.02 +/- 0.04 (calf, mean +/- SD, n = 7) and 1.04 +/- 0.11 (adult bovine, n = 8). Sodium concentration as measured by NMR was then used with ideal Donnan theory to compute estimates of FCD. For calf articular cartilage (AC) near physiological conditions, calculated FCD was -0.28 +/- 0.03 M (n = 10). NMR measurements were then made for individual cartilage specimens sequentially equilibrated in baths of differing salt composition, pH, or ionic strength. For calf and adult AC, calculated FCD decreased dramatically between pH 3 and 2, with adult specimens becoming positively charged but calf tissue retaining a net negative charge. For calf AC equilibrated in 0.3-0.015 M NaCl, calculated FCD was observed to decrease slightly with decreasing bath ionic strength. For epiphyseal cartilage, FCD varied with the position of origin of the explant within the joint, ranging from -0.19 to -0.35 M in a manner that correlated with tissue glycosaminoglycan content. Preliminary NMR imaging experiments demonstrated similar variations of sodium concentration in intact ulnar epiphyseal cartilage. Collectively, these results demonstrate the ability of NMR to nondestructively follow FCD in cartilage. The technique is applicable to dynamic studies as well as to both in vitro and in vivo studies on living tissue.