Clinicopathological significance of the gene expression of matrix metalloproteinase-7, insulin-like growth factor-1, insulin-like growth factor-2 and insulin-like growth factor-1 receptor in patients with colorectal cancer:: Insulin-like growth factor-1 receptor gene expression is a useful predictor of liver metastasis from colorectal cancer
Clinicopathological significance of the gene expression of matrix metalloproteinase-7, insulin-like growth factor-1, insulin-like growth factor-2 and insulin-like growth factor-1 receptor in patients with colorectal cancer:: Insulin-like growth factor-1 receptor gene expression is a useful predictor of liver metastasis from colorectal cancer
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DOI：
10.3892/or_00000015
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发表时间：
2008-08-01
期刊：
影响因子：
4.2
通讯作者：
Imada, Toshio
中科院分区：
文献类型：
--
作者：
Oshima, Takashi;Akaike, Makoto;Imada, Toshio
基质金属蛋白酶 -7（MMP -7）由癌细胞分泌，传统上一直与肿瘤细胞侵袭和转移相关的基底膜破坏有关。流行病学研究已经确定循环中高水平的胰岛素样生长因子 -1（IGF -1）与结直肠癌的相对风险之间存在相关性，已知结直肠癌会产生MMP -7。我们研究了MMP -7、IGF -1、IGF -2和IGF -1受体基因在结直肠癌患者中的相对表达的临床病理意义，特别是关于转移方面。我们研究了205例未经治疗的结直肠癌患者的癌组织和邻近正常黏膜的手术标本。通过实时定量逆转录聚合酶链反应测量癌组织和邻近正常黏膜中的MMP -7、IGF -1、IGF -2、IGF -1R和β - 肌动蛋白mRNA。癌组织中MMP -7和IGF -1R基因表达水平高于邻近正常黏膜。相反，IGF -1基因表达在癌组织中低于邻近正常黏膜。至于基因表达与临床病理因素的关系，IGF -1R表达与静脉侵犯和肝转移相关。因此，IGF -1R基因表达被认为是结直肠癌肝转移的一个有用的预测指标。
Matrix metalloproteinase-7 (MMP-7), secreted by cancer cells, has been implicated classically in the basement membrane destruction associated with tumor cell invasion and metastasis. Epidemiological studies have established a correlation between high levels of circulating insulin-like growth factor-1 (IGF-1) and the relative risk of colorectal cancer, which is known to produce MMP-7. We examined the clinicopathological significance of the relative expression of MMP-7, IGF-1, IGF-2 and IGF-1 receptor genes in patients with colorectal cancer, especially with regard to metastasis. We studied surgical specimens of cancer tissue and adjacent normal mucosa obtained from 205 patients with untreated colorectal carcinoma. MMP-7, IGF-1, IGF-2, IGF-1R and beta-actin mRNA in cancer tissue and adjacent normal mucosa were measured by quantitative real-time reverse-transcriptase polymerase chain reaction. MMP-7 and IGF-1R gene expression levels were higher in cancer tissue than in adjacent normal mucosa. In contrast, IGF-1R gene expression was lower in cancer tissue than in adjacent normal mucosa. As for the relationship of gene expression to clinicopathological factors, 1GF-1R expression correlated with venous invasion and liver metastasis. IGF-1R gene expression is thus considered a useful predictor of liver metastasis from colorectal cancer.