Virus Reduction during Advanced Bardenpho and Conventional Wastewater Treatment Processes
Virus Reduction during Advanced Bardenpho and Conventional Wastewater Treatment Processes
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DOI：
10.1021/acs.est.6b01384
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发表时间：
2016-09-06
影响因子：
11.4
通讯作者：
Pepper, Ian L.
中科院分区：
文献类型：
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作者：
Schrnitz, Bradley W.;Kitajima, Masaaki;Pepper, Ian L.
本研究调查了废水处理去除11种不同的病毒类型（辣椒温和斑驳病毒，爱知病毒，基因组I，II，IV诺如病毒，肠道病毒，sapovirus，A组轮状病毒，腺病毒，JC和BK多瘤病毒）的两个废水处理设施，利用先进的Bardenpho技术，并与传统的处理工艺的结果进行了比较。据我们所知，这是第一次研究比较全规模的处理过程，所有接收来自同一地区的污水进水。废水中的病毒的发病率进行了评估的绝对丰度，发生率，并在整个12个月期间在亚利桑那州南部每月收集的样本减少。通过负电性过滤器方法浓缩样品，并使用基于TaqMan的定量聚合酶链反应（qPCR）进行定量。结果表明，工厂D，利用先进的Bardenpho工艺作为二级处理，有效地减少了病原病毒比设施使用传统工艺。然而，由于缺乏细胞培养试验，无法对感染性病毒进行准确评估。根据这些数据，爱知病毒被认为是一种保守的病毒标志物，用于适当的废水处理，因为它最常显示出与病毒病原体的最佳相关系数，总是在较高浓度下检测到，并且可能高估潜在的病毒风险。
The present study investigated wastewater treatment for the removal of 11 different virus types (pepper mild mottle virus; Aichi virus; genogroup I, II, and IV noroviruses; enterovirus; sapovirus; group-A rotavirus; adenovirus; and JC and BK polyomaviruses) by two wastewater treatment facilities utilizing advanced Bardenpho technology and compared the results with conventional treatment processes. To our knowledge, this is the first study comparing full-scale treatment processes that all received sewage influent from the same region. The incidence of viruses in wastewater was assessed with respect to absolute abundance, occurrence, and reduction in monthly samples collected throughout a 12 month period in southern Arizona. Samples were concentrated via an electronegative filter method and quantified using TaqMan-based quantitative polymerase chain reaction (qPCR). Results suggest that Plant D, utilizing an advanced Bardenpho process as secondary treatment, effectively reduced pathogenic viruses better than facilities using conventional processes. However, the absence of cell-culture assays did not allow an accurate assessment of infective viruses. On the basis of these data, the Aichi virus is suggested as a conservative viral marker for adequate wastewater treatment, as it most often showed the best correlation coefficients to viral pathogens, was always detected at higher concentrations, and may overestimate the potential virus risk.