Single argonaute protein from Toxoplasma gondii is involved in the double-stranded RNA induced gene silencing
Single argonaute protein from Toxoplasma gondii is involved in the double-stranded RNA induced gene silencing
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DOI：
10.1016/j.ijpara.2006.04.014
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发表时间：
2006-08-01
影响因子：
4
通讯作者：
Ananvoranich, Sirinart
中科院分区：
文献类型：
--
作者：
Al Riyahi, Ahmad;Al-Anouti, Fatme;Ananvoranich, Sirinart
在这里，我们报告了弓形虫 argonaute 蛋白的特征。这是关于古菌和真核生物的藻氨酸蛋白的结构特征重叠的藻氨酸蛋白的功能的第一份报告。全长cDNA克隆具有1575 bp的开放阅读框，编码524个氨基酸的蛋白质，计算分子量为58.5 kDa，估计等电点为9.4。这种称为TgAgo的argonaute蛋白具有独特的特征：（i）TgAgo比报道的源自高等真核生物（即拟南芥、人类和线虫）的argonaute蛋白小，但与古细菌（即激烈火球菌和Archaeoglobus fulgidus）的argonaute蛋白大小相似； (ii) TgAgo包含保守的PIWI结构域和非保守的PAZ结构域； (iii) TgAgo 主要定位于细胞质； (iv) 尽管 TgAgo 体积小，但它参与双链 RNA 诱导的基因沉默。使用 TgAgo 表达降低的转基因寄生虫系，我们发现 TgAgo 的表达是双链 RNA 诱导的基因沉默、RNA 干扰机制所必需的。 (c) 2006 年澳大利亚寄生虫学协会。由 Elsevier Ltd 出版。保留所有权利。
Here, we report the characterization of the argonaute protein from Toxoplasma gondii. This is the first report on the function of an argonaute protein with structural features overlapping between argonaute proteins of archaeal bacteria and eukaryotes. The full-length cDNA clone has an open reading frame of 1575 bp, which encodes a 524 amino acid protein with a calculated molecular weight of 58.5 kDa and an estimated isoelectric point of 9.4. This argonaute protein, called TgAgo, exhibits unique features: (i) TgAgo is smaller than reported argonaute proteins derived from higher eukaryotic organisms (i.e. Arabidopsis, human and nematodes) but has a similar size to those from archaeal bacteria (i.e. Pyrococcus furiosus and Archaeoglobus fulgidus); (ii) TgAgo contains a conserved PIWI domain and non-conserved PAZ domain; (iii) TgAgo is mainly localized in the cytoplasm; and (iv) despite its small size, TgAgo participates in the double-stranded RNA induced gene silencing. Using a transgenic parasite line, in which TgAgo expression is lowered, we showed that the expression of TgAgo is required for the double-stranded RNA induced gene silencing, RNA interference mechanism. (c) 2006 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.