Enzymes in the juvenile hormone biosynthetic pathway can be potential targets for pest control
Enzymes in the juvenile hormone biosynthetic pathway can be potential targets for pest control
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保幼激素生物合成途径中的酶可以成为害虫防治的潜在目标
DOI：
10.1002/ps.5617
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发表时间：
2019
影响因子：
4.1
通讯作者：
Zhen-Peng Kai
中科院分区：
文献类型：
--
作者：
Yue Yin;Yan-wei Qiu;Juan Huang;Stephen S Tobe;Shan-Shan Chen;Zhen-Peng Kai
近年来，新杀虫剂和靶标的发现受到全球关注。以烟草天蛾（Manducasexta）保幼激素生物合成途径中的10个关键酶基因为研究对象，测定了保幼激素生物合成途径中关键酶基因的表达。根据基因表达结果，选择法尼醇脱氢酶（FOLD）、保幼激素酸性O-甲基转移酶（JHAMT）和保幼激素环氧酶（CYP 15 C1）作为候选靶标。通过RNAi沉默和酶抑制剂试验验证这些候选基因是否可能成为潜在的杀虫剂靶标。FOLD、JHAMT和CYP 15 C1的下调分别导致体外JH生物合成率降低68%、82%和79%。此外，这些酶的RNA干扰和抑制剂的研究表明，口服给药在害虫管理的潜在应用，在高死亡率和对growth.CONCLUSIONBased上我们的研究，FOLD，JHAMT和CYP 15 C1可能是潜在的目标害虫控制的结果，其在昆虫发育中的重要作用。© 2019化学工业协会
BACKGROUNDDiscovery of novel insecticides and targets has received global attention in recent years. Ten genes coding for enzymes involved in the juvenile hormone biosynthetic pathway ofManduca sextawere studied as potential insecticide targets.RESULTSWe determined the expression of genes encoding some critical enzymes in the JH biosynthetic pathway. Farnesol dehydrogenase (FOLD), Juvenile hormone acid O‐methyltransferase (JHAMT) and Juvenile hormone epoxidase (CYP15C1) were selected as the candidate targets based on gene expression results. RNAi silencing and enzyme inhibitor tests were performed to validate whether these candidate genes could be the potential insecticide targets. The down‐regulation ofFOLD,JHAMTandCYP15C1resulted in a 68%, 82% and 79% reduction in the rates of JH biosynthesisin vitro, respectively. In addition, RNA interference and inhibitor studies of these enzymes following oral administration demonstrated the potential application in pest management, with respect to high mortality and effects on growth.CONCLUSIONBased on our study, FOLD, JHAMT and CYP15C1 could be potential targets for pest control as a consequence of their important roles in insect development. © 2019 Society of Chemical Industry