Prolonged detection of indigenous wild polioviruses in sewage from communities in Egypt
Prolonged detection of indigenous wild polioviruses in sewage from communities in Egypt
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DOI：
10.1093/aje/kwg202
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发表时间：
2003-10-15
影响因子：
5
通讯作者：
Wahdan, MH
中科院分区：
文献类型：
--
作者：
El Bassioni, L;Barakat, I;Wahdan, MH
埃及已对脊髓灰质炎病毒进行了环境监测。本文报道了一项研究，其中130个污水样品收集于2001年1月至2001年12月从埃及的八个省。通过L20 B和RD细胞培养物中的病毒分离来分析样品，并且通过VP1蛋白编码区的测序来表征野生脊髓灰质炎病毒。在57%的污水样本和91%的研究地点中检测到野生1型脊髓灰质炎病毒，其中只有两个地点在2001年报告了麻痹性脊髓灰质炎病例。在污水中可检测到单一本土1型脊髓灰质炎病毒基因型的三个遗传谱系，通过对急性弛缓性麻痹的监测也仅检测到一个谱系。尽管开展了口服脊髓灰质炎病毒疫苗免疫接种运动，但野生脊髓灰质炎病毒仍然存在于环境中。建议后续活动包括继续分析污水样本、严格评价免疫覆盖率和监测急性弛缓性麻痹。
Environmental surveillance for polioviruses has been implemented in Egypt. This paper reports on a study in which 130 sewage samples were collected between January 2001 and December 2001 from eight provinces of Egypt. Samples were analyzed by virus isolation in L20B and RD cell cultures, and wild polioviruses were characterized by sequencing of the VP1 protein coding region. Wild type 1 polioviruses were detected in 57% of the sewage samples and 91% of the study sites, only two of which reported paralytic poliomyelitis cases in 2001. Three genetic lineages of a single indigenous type 1 poliovirus genotype were detectable in sewage, and only one lineage was also detected through surveillance for acute flaccid paralysis. Wild polioviruses persisted in the environment despite implementation of oral poliovirus vaccine immunization campaigns. Continued analysis of sewage samples, critical evaluation of immunization coverage, and performance of surveillance for acute flaccid paralysis are proposed as follow-up activities.