Coupling carbon nanotube film microextraction with desorption corona beam ionization for rapid analysis of Sudan dyes (I-IV) and Rhodamine B in chilli oil
Coupling carbon nanotube film microextraction with desorption corona beam ionization for rapid analysis of Sudan dyes (I-IV) and Rhodamine B in chilli oil
复制标题
碳纳米管膜微萃取与解吸电晕束电离耦合快速分析辣椒油中的苏丹染料 (I-IV) 和罗丹明 B。
DOI：
10.1039/c4an02044h
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发表时间：
2015-01-01
期刊：
影响因子：
4.2
通讯作者：
Feng, Yu-Qi
中科院分区：
文献类型：
--
作者：
Chen, Di;Huang, Yun-Qing;Feng, Yu-Qi
建立了碳纳米管膜（CNTF）微萃取-解吸电晕束离子化（DCBI）快速分析辣椒油中苏丹红（I-IV）和罗丹明B的方法。通常，CNTF被浸入辣椒油的稀释溶液中进行提取，然后将其直接放置在DCBI源的可见等离子体束尖端下进行解吸和电离。在优化的条件下，用该方法同时测定了5种染料。结果表明，CNTF通过π-π相互作用富集被测物，与DCBI-MS/MS直接分析相比，该方法的灵敏度显著提高。在多反应监测模式下，CNTF的线性范围为0.08-12.8 μg g（-1），相关系数R2> 0.93。获得了令人满意的重现性。相对标准偏差（RSD）小于20.0%。方法的回收率为80.0 ~ 110.0%，检出限为1.4 ~ 21 ng g（-1）。最后，通过对辣椒粉中5种违禁染料的测定，进一步证明了该方法的可行性。这些结果表明，该方法消耗的时间和溶剂比传统的HPLC为基础的方法，并避免了色谱柱和离子源的非挥发性油的污染。在72孔摇床的帮助下，可以同时处理多个样品，从而确保整个预处理过程的高通量。总之，它提供了一个快速和高通量的方法来确定辣椒产品中的非法添加。
A rapid analysis method by coupling carbon nanotube film (CNTF) microextraction with desorption corona beam ionization (DCBI) was developed for the determination of Sudan dyes (I-IV) and Rhodamine B in chilli oil samples. Typically, CNTF was immersed into the diluted solution of chilli oil for extraction, which was then placed directly under the visible plasma beam tip of the DCBI source for desorption and ionization. Under optimized conditions, five dyes were simultaneously determined using this method. Results showed that the analytes were enriched by the CNTF through the π-π interactions, and the proposed method could significantly improve the sensitivities of these compounds, compared to the direct analysis by DCBI-MS/MS. The method with a linear range of 0.08-12.8 μg g(-1) and good linear relationships (R(2) > 0.93) in a multiple reaction monitoring (MRM) mode was developed. Satisfactory reproducibility was achieved. Relative standard deviations (RSDs) were less than 20.0%. The recoveries ranged from 80.0 to 110.0%, and the limits of detection (LODs) were in the range of 1.4-21 ng g(-1). Finally, the feasibility of the method was further exhibited by the determination of five illegal dyes in chilli powder. These results demonstrate that the proposed method consumes less time and solvent than conventional HPLC-based methods and avoids the contamination of chromatographic column and ion source from non-volatile oil. With the help of a 72-well shaker, multiple samples could be treated simultaneously, which ensures high throughput for the entire pretreatment process. In conclusion, it provides a rapid and high-throughput approach for the determination of such illicit additions in chilli products.