A water-sprayable, thermogelating and biocompatible polymer host for use in fluorescent chemical sensing and imaging of oxygen, pH values and temperature
A water-sprayable, thermogelating and biocompatible polymer host for use in fluorescent chemical sensing and imaging of oxygen, pH values and temperature
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DOI：
10.1016/j.snb.2015.05.082
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发表时间：
2015-12
影响因子：
8.4
通讯作者：
Xu-dong Wang;R. Meier;Carina Schmittlein;S. Schreml;M. Schäferling;O. Wolfbeis
中科院分区：
文献类型：
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作者：
Xu-dong Wang;R. Meier;Carina Schmittlein;S. Schreml;M. Schäferling;O. Wolfbeis
我们报告了可喷雾热凝胶生物材料（Poloxamer™；又名 Pluronic™）在 pH 值、局部氧气和温度的光学成像中的使用。该材料具有高度生物相容性且易于处理。我们还表明，该材料具有良好的氧气渗透性（因此使其成为氧气传感器的良好选择），并且在液体溶液和高温下稳定。我们展示了其在氧气、pH 值和温度光学传感器中的适用性。这是通过将适当的发光探针掺入各种微粒（充当探针的主体并防止染料浸出和聚集），然后将微粒分散在热凝胶聚合物中来实现的。所得传感器凝胶通过在<20°C 的温度下喷涂沉积在感兴趣的表面上。在这些温度下，即使在皮肤、伤口和细菌培养物等不平坦的表面上，凝胶也能很好地粘附在目标上。如果温度升至 25°C 以上，凝胶会形成一层薄而柔软但坚固的传感层，但是可以通过冷却并擦拭或用水清洗来简单地将其从感兴趣的表面上去除。与其他传感器相比，可喷涂热凝胶传感器具有明显的优势，不会对目标表面造成损坏。在我们看来，传感材料在其他物种的传感器中也具有广泛的进一步适用性，包括临床相关气体、酶底物（如葡萄糖或乳酸）和离子。
We report on the use of a sprayable and thermogelating biomaterial (Poloxamer™; a.k.a. Pluronic™) in optical imaging of pH values, local oxygen and temperature. The material is highly biocompatible and easy to handle. We also show that the material is well permeable to oxygen (thus making it a good choice for use in oxygen sensors), and is stable in liquid solution and at elevated temperature. We demonstrate its applicability in optical sensors for oxygen, pH and temperature. This was accomplished by incorporating appropriate luminescent probes in various kinds of microparticles (which act as hosts for the probes and prevent dye leaching and aggregation), and then dispersing the microparticles in the thermogelating polymer. The resulting sensor gels were deposited on the surface of interest via spraying at temperatures of <20 °C. At these temperatures, the gels adhere well to the target, even on uneven surfaces such as skin, wounds, and bacterial cultures. If temperature is risen to above 25 °C, the gels form a thin and soft but solid sensing layer which, however, can be simply removed from surface of interest by cooling and wiping it off, or by washing with water. Sprayable thermogelating sensors present obvious advantages over other sensors by not causing damage to the surface of interest. In our perception, the sensing materials also have wide further applicability in sensors for other species including clinically relevant gases, enzyme substrates (such as glucose or lactate) and ions.