Stomatal Development and Gene Expression in Rice Florets.
Stomatal Development and Gene Expression in Rice Florets.
复制标题
水稻小花的气孔发育和基因表达。
DOI：
10.1093/pcp/pcac120
复制
发表时间：
2022
影响因子：
4.9
通讯作者：
Bertolino LT
中科院分区：
文献类型：
--
作者：
Bertolino LT
气孔在调节植物与大气之间的气体交换中起着重要作用。这些显微结构在叶表皮上大量形成，也存在于花上。虽然叶气孔的研究已经很深入，但花气孔的发育和功能却很少受到关注。在这里，我们详细描述了籼稻品种IR 64的花气孔的空间分布和发展。我们发现，气孔复合体是目前在低密度的外稃，内稃和花药的特定区域，并在形态上不同的叶片上发现的气孔。我们发现，在苞片状器官中，气孔发育遵循与水稻叶片相同的细胞谱系转换，并证明气孔发育调节因子OsEPFL 9 - 1和OsEPF 1的过表达导致水稻花器官中气孔密度的显著变化，产生约两倍于气孔的外稃（OsEPFL 9 -1_oe）或几乎没有气孔的外稃（OsEPF1_oe）。对发育中小花的转录组学分析也表明，花气孔发育过程中的细胞转换受到与水稻叶片相同的遗传网络的调控。最后，虽然我们无法检测到与花气孔密度变化相关的植物繁殖影响，但我们报告了过表达OsEPF 1的株系中全局基因表达的改变，并讨论了我们的结果如何反映花气孔的可能作用。
Stomata play a fundamental role in modulating the exchange of gases between plants and the atmosphere. These microscopic structures form in high numbers on the leaf epidermis and are also present on flowers. Although leaf stomata are well studied, little attention has been paid to the development or function of floral stomata. Here, we characterize in detail the spatial distribution and development of the floral stomata of the indica rice variety IR64. We show that stomatal complexes are present at low density on specific areas of the lemma, palea and anthers and are morphologically different compared to stomata found on leaves. We reveal that in the bract-like organs, stomatal development follows the same cell lineage transitions as in rice leaves and demonstrate that the overexpression of the stomatal development regulatorsOsEPFL9-1andOsEPF1leads to dramatic changes in stomatal density in rice floral organs, producing lemma with approximately twice as many stomata (OsEPFL9-1_oe) or lemma where stomata are practically absent (OsEPF1_oe). Transcriptomic analysis of developing florets also indicates that the cellular transitions during the development of floral stomata are regulated by the same genetic network used in rice leaves. Finally, although we were unable to detect an impact on plant reproduction linked to changes in the density of floral stomata, we report alterations in global gene expression in lines overexpressingOsEPF1and discuss how our results reflect on the possible role(s) of floral stomata.