Differential expression of decorin and biglycan genes during mouse tooth development
Differential expression of decorin and biglycan genes during mouse tooth development
复制标题
DOI：
10.1016/s0945-053x(01)00142-1
复制
发表时间：
2001-09-01
期刊：
影响因子：
6.9
通讯作者：
Yamauchi, M
中科院分区：
文献类型：
--
作者：
Matsuura, T;Duarte, WR;Yamauchi, M
富含亮氨酸的小分子蛋白聚糖（SLRP）具有多种生物学功能，其中一些被认为是调节骨和牙齿中胶原矿化的蛋白。我们以前已经确定和免疫定位的SLRPs家族，核心蛋白聚糖和biglycan，在牛牙齿/牙周组织的两个成员。为了研究它们在牙齿发育中的潜在作用，我们通过原位杂交检测了新生（19天）小鼠牙胚中核心蛋白聚糖、双糖链蛋白聚糖和I型胶原的mRNA表达模式。在这个发育阶段，上颌第一磨牙和下颌第一磨牙分别包括前牙本质基质分泌之前和之后的阶段。核心蛋白聚糖mRNA的表达与I型胶原mRNA的表达一致，主要见于分泌期的成牙本质细胞，而双糖蛋白聚糖mRNA在整个牙胚中均有表达，包括分泌期前的成牙本质细胞/成釉细胞、牙乳头和星网状细胞。然而，它的信号在分泌型成牙本质细胞是不明显的核心蛋白聚糖。在下颌切牙中，已经分泌了大量的前牙本质基质和少量的釉质基质，观察到类似的差异表达模式。在分泌期成釉细胞中，双糖蛋白聚糖mRNA表达明显，而核心蛋白聚糖mRNA表达不明显。这些差异表达模式表明，双糖链蛋白聚糖和核心蛋白聚糖在牙齿发育过程中的不同作用。(C)2001 Elsevier Science B.V./国际矩阵生物学学会。All rights reserved.
Small leucine-rich proteoglycans (SLRPs) have a number of biological functions and some of them are thought to regulate collagen mineralizaton in bone and tooth. We have previously identified and immunolocalized two members of the SLRPs family, decorin and biglycan, in bovine tooth/periodontium. To investigate their potential roles in tooth development, we examined the mRNA expression patterns of decorin, biglycan and type I collagen in newborn (day 19) mice tooth germs by in situ hybridization. At this developmental stage, the first maxillary and mandibular molars include stages before and after secretion of the predentin matrix, respectively. The expression of decorin mRNA coincided with that of type I collagen mRNA and was mostly observed in secretory odontoblasts, while the biglycan mRNA was expressed throughout the tooth germ, including pre-secretory odontoblasts/ameloblasts, dental papilla and stellate reticulum. However, its signal in secretory odontoblasts was not as evident as that of decorin. In mandibular incisors, where a significant amount of predentin matrix and a small amount of enamel matrix were already secreted, a similar differential expression pattern was observed. In secretory ameloblasts the biglycan mRNA expression was apparent, while that of decorin was not. These differential expression patterns suggest the distinct roles of biglycan and decorin in the process of tooth development. (C) 2001 Elsevier Science B.V./International Society of Matrix Biology. All rights reserved.