A PHOSPHATE TRANSPORTER FROM THE MYCORRHIZAL FUNGUS GLOMUS VERSIFORME
A PHOSPHATE TRANSPORTER FROM THE MYCORRHIZAL FUNGUS GLOMUS VERSIFORME
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DOI：
10.1038/378626a0
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发表时间：
1995-12-07
期刊：
影响因子：
64.8
通讯作者：
VANBUUREN, ML
中科院分区：
文献类型：
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作者：
HARRISON, MJ;VANBUUREN, ML
泡状丛枝菌根真菌与大多数陆生植物的根形成共生关系，包括许多在农业上重要的作物物种(1-3)。真菌在根部的皮层上定居，从它们的植物宿主那里获得碳，同时帮助植物从土壤中吸收磷酸盐和其他矿物质营养(2-5)。这种结合对植物是有利的，因为磷对植物的生长和发育是必不可少的，特别是在营养限制条件下的生长期间(2，6-8)。由于VA真菌专性共生的性质，对这些真菌及其与植物相互作用的分子遗传学研究一直是有限的，因此真菌介导的磷从土壤到植物吸收和转运的分子机制仍不清楚。在这里，我们通过从VA菌根真菌Glomus versiforme中鉴定编码跨膜磷酸转运蛋白(GvPT)的互补DNA来研究这一过程。通过与酵母磷酸转运蛋白突变体的互补，证实了GvPT编码蛋白的功能。在菌根联合过程中，GvPT的表达定位于菌根真菌的外部菌丝，这是从土壤中吸收磷的初始位置。
VESICULAR-arbuscular (A) mycorrhizal fungi form symbiotic associations with the roots of most terrestrial plants, including many agriculturally important crop species(1-3). The fungi colonize the cortex of the root to obtain carbon from their plant host, while assisting the plant with the uptake of phosphate and other mineral nutrients from the soil(2-5). This association is beneficial to the plant, because phosphate is essential for plant growth and development, especially during growth under nutrient-limiting conditions(2,6-8). Molecular genetic studies of these fungi and their interaction with plants have been limited owing to the obligate symbiotic nature of the VA fungi, so the molecular mechanisms underlying fungal-mediated uptake and translocation of phosphate from the soil to the plant remain unknown. Here we begin to investigate this process by identifying a complementary DNA that encodes a transmembrane phosphate transporter (GvPT) from Glomus versiforme, a VA mycorrhizal fungus. The function of the protein encoded by GvPT was confirmed by complementation of a yeast phosphate transport mutant. Expression of GvPT was localized to the external hyphae of G. versiforme during mycorrhizal associations, these being the initial site of phosphate uptake from the soil.