Screening of dietary antioxidants against mitochondria-mediated oxidative stress by visualization of intracellular redox state
Screening of dietary antioxidants against mitochondria-mediated oxidative stress by visualization of intracellular redox state
复制标题
通过细胞内氧化还原状态的可视化筛选抗线粒体介导的氧化应激的膳食抗氧化剂
DOI：
10.1080/09168451.2015.1123607
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Y. Sakai and J. Hoseki
中科院分区：
文献类型：
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作者：
S. Maharjan;Y. Sakai and J. Hoseki
线粒体损伤和由此产生的活性氧（ROS）与衰老及其相关的病理条件。最近，膳食抗氧化剂作为潜在的预防和治疗剂对ROS产生的衰老和病理条件获得了显着的关注。我们之前证明，食物来源的抗氧化剂可以在蛋白酶体抑制条件下预防细胞内氧化应激，这归因于线粒体功能障碍和活性氧产生，然后导致细胞死亡。在这里，我们进一步筛选饮食抗氧化剂的活性作为氧化还原调节剂的氧化还原状态的可视化使用Redoxfluor，荧光蛋白氧化还原探针。抗氧化剂直接缓解ROS，但不诱导抗氧化酶，阻止了细胞内氧化。有效的抗氧化剂清除线粒体ROS并抑制细胞死亡。我们的研究表明，氧化还原可视化下的线粒体介导的氧化应激是有用的筛选潜在的抗氧化剂，以抵消线粒体功能障碍，这已牵连在衰老和衰老相关疾病的发病机制。
Mitochondrial impairment and the resulting generation of reactive oxygen species (ROS) have been associated with aging and its related pathological conditions. Recently, dietary antioxidants have gained significant attention as potential preventive and therapeutic agents against ROS-generated aging and pathological conditions. We previously demonstrated that food-derived antioxidants prevented intracellular oxidative stress under proteasome inhibition conditions, which was attributed to mitochondrial dysfunction and ROS generation, followed by cell death. Here, we further screened dietary antioxidants for their activity as redox modulators by visualization of the redox state using Redoxfluor, a fluorescent protein redox probe. Direct alleviation of ROS by antioxidants, but not induction of antioxidative enzymes, prevented mitochondria-mediated intracellular oxidation. The effective antioxidants scavenged mitochondrial ROS and suppressed cell death. Our study indicates that redox visualization under mitochondria-mediated oxidative stress is useful for screening potential antioxidants to counteract mitochondrial dysfunction, which has been implicated in aging and the pathogenesis of aging-related diseases.