Enrichment and Characterization of a Psychrotolerant Consortium Degrading Crude Oil Alkanes Under Methanogenic Conditions
Enrichment and Characterization of a Psychrotolerant Consortium Degrading Crude Oil Alkanes Under Methanogenic Conditions
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DOI：
10.1007/s00248-015-0590-y
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发表时间：
2015-08-01
影响因子：
3.6
通讯作者：
Zhang, Hui
中科院分区：
文献类型：
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作者：
Ding, Chen;Ma, Tingting;Zhang, Hui
在中温和高温条件下，通过产甲烷来厌氧降解烷烃已被广泛研究。然而，关于低温条件下厌氧烷烃降解微生物群落的能力和组成的研究却很少。研究了胜利油田富集的Y15菌群在不同温度条件下（5-35 A ℃）对烃类的降解能力。该联合体可以在低温范围（15-30摄氏度）内使用十六烷。在低于10 A ℃和高于35 A ℃时未检测到生长，表明存在能够降解烷烃的耐寒物种。该菌群对原油中的短链正构烷烃具有优先降解作用。利用末端限制性片段长度多态性（T-RFLP）指纹图谱和16 S rRNA基因的桑格测序研究了微生物群落的结构和动态。核心古菌群落主要由醋酸菌Methanosaeta spp.在连续转移过程中总是检测到与互养菌科相关的微生物，并在细菌群落中占主导地位，与丙酸史密斯氏菌菌株LYPT具有94- 96%的序列相似性。系统发育分析显示，在不同的产甲烷烷烃降解文化的Syntrophaceae相关克隆，其中大多数被聚成3个亚系。从这项研究中检索到的互养科克隆主要聚类成亚系I，这可能代表psychrotolerant，互养烷烃降解。这些结果表明，广泛的地理分布和生态功能的互养烷烃降解菌。
Anaerobic alkane degradation via methanogenesis has been intensively studied under mesophilic and thermophilic conditions. While there is a paucity of information on the ability and composition of anaerobic alkane-degrading microbial communities under low temperature conditions. In this study, we investigated the ability of consortium Y15, enriched from Shengli oilfield, to degrade hydrocarbons under different temperature conditions (5-35 A degrees C). The consortium could use hexadecane over a low temperature range (15-30 A degrees C). No growth was detected below 10 A degrees C and above 35 A degrees C, indicating the presence of cold-tolerant species capable of alkane degradation. The preferential degradation of short chain n-alkanes from crude oil was observed by this consortium. The structure and dynamics of the microbial communities were examined using terminal restriction fragment length polymorphism (T-RFLP) fingerprinting and Sanger sequencing of 16S rRNA genes. The core archaeal communities were mainly composed of aceticlastic Methanosaeta spp. Syntrophaceae-related microorganisms were always detected during consecutive transfers and dominated the bacterial communities, sharing 94-96 % sequence similarity with Smithella propionica strain LYPT. Phylogenetic analysis of Syntrophaceae-related clones in diverse methanogenic alkane-degrading cultures revealed that most of them were clustered into three sublineages. Syntrophaceae clones retrieved from this study were mainly clustered into sublineage I, which may represent psychrotolerant, syntrophic alkane degraders. These results indicate the wide geographic distribution and ecological function of syntrophic alkane degraders.