Grazing of leaf-associated Cercomonads (Protists: Rhizaria: Cercozoa) structures bacterial community composition and function
Grazing of leaf-associated Cercomonads (Protists: Rhizaria: Cercozoa) structures bacterial community composition and function
复制标题
DOI：
10.1111/1462-2920.13824
复制
发表时间：
2017-08-01
影响因子：
5.1
通讯作者：
Bonkowski, Michael
中科院分区：
文献类型：
--
作者：
Flues, Sebastian;Bass, David;Bonkowski, Michael
原生生物的食物选择偏好是有据可查的，但我们仍然缺乏基本的了解原生生物捕食如何改变细菌群落的分类和功能组成。我们进行了饲养试验，使用叶相关的尾孢单胞菌Cercozoa孵育他们在一个标准化的，多样化的细菌群落洗从植物叶片。我们使用鸟枪宏基因组学的方法来研究细菌群落的分类和功能的变化后，五天的原生生物捕食细菌。捕食引起的细菌群落组成的变化可能与表型原生生物性状有关。原生生物的繁殖率、形态可塑性和细胞速度是决定细菌群落组成的重要因素。共生模式的分析表明，不太复杂的相关性细菌类群之间的原生动物放牧治疗的正相关性比非放牧对照的比例较高，这表明捕食减少了强大的竞争对手的影响。原生生物捕食影响14代谢的核心功能，包括膜运输VI型分泌系统，特别是上调。鉴于植物叶际和根际细菌群落的功能重要性，显然需要更详细地了解捕食者-猎物的相互作用，微生物组成和功能的变化，以及随后对植物性能的影响。
Preferential food selection in protists is well documented, but we still lack basic understanding on how protist predation modifies the taxonomic and functional composition of bacterial communities. We conducted feeding trials using leaf-associated cercomonad Cercozoa by incubating them on a standardized, diverse bacterial community washed from plant leaves. We used a shotgun metagenomics approach to investigate the taxonomic and functional changes of the bacterial community after five days protist predation on bacteria. Predation-induced shifts in bacterial community composition could be linked to phenotypic protist traits. Protist reproduction rate, morphological plasticity and cell speed were most important in determining bacterial community composition. Analyses of co-occurrence patterns showed less complex correlations between bacterial taxa in the protist-grazed treatments with a higher proportion of positive correlations than in non-grazed controls, suggesting that predation reduced the influence of strong competitors. Protist predation influenced 14 metabolic core functions including membrane transport from which type VI secretion systems were in particular upregulated. In view of the functional importance of bacterial communities in the phyllosphere and rhizosphere of plants, a more detailed understanding of predator-prey interactions, changes in microbial composition and function, and subsequent repercussions on plant performance are clearly required.