Induction of hepatocyte growth factor production in human dermal fibroblasts and their proliferation by the extract of bitter melon pulp
Induction of hepatocyte growth factor production in human dermal fibroblasts and their proliferation by the extract of bitter melon pulp
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DOI：
10.1016/j.cyto.2008.12.016
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发表时间：
2009-04-01
期刊：
影响因子：
3.8
通讯作者：
Gohda, Eiichi
中科院分区：
文献类型：
--
作者：
Ono, Takehiro;Tsuji, Tomoe;Gohda, Eiichi
肝细胞生长因子（HGF）通过诱导上皮细胞和内皮细胞增殖而成为治疗肝、肾纤维化和心血管疾病的潜在药物。HGF诱导剂也可用作这些疾病的治疗剂。然而，还没有关于通过植物提取物或汁液诱导HGF产生的报道。苦瓜（Momorea charantia L.）牙髓显著诱导HGF的产生。有一个时间滞后72小时诱导后，提取物添加HGF生产。其刺激作用伴随着HGF基因表达的上调。增加有丝分裂原活化蛋白激酶（MAPKs），观察到从72小时后，提取物添加。MAPKs抑制剂抑制提取物诱导的HGF产生。该提取物还刺激细胞增殖。诱导HGF产生和细胞增殖的两种活性在凝胶过滤上以14，000Da的单峰一起洗脱。结果表明苦瓜果肉提取物诱导人皮肤成纤维细胞产生HGF和细胞增殖，并提示HGF诱导过程中参与了TGF β 1的激活。我们的研究结果表明，提取物的组织再生的潜在用途，并提供了一个深入了解苦瓜的伤口愈合特性的分子机制。(C)2009爱思唯尔有限公司保留所有权利。
Hepatocyte growth factor (HGF) is useful as a potential therapeutic agent for hepatic and renal fibrosis and cardiovascular diseases through inducing proliferation of epithelial and endothelial cells. HGF inducers may also be useful as therapeutic agents for these diseases. However, there have been no reports on induction of HGF production by plant extracts or juices. An extract of bitter melon (Momordica charantia L.) pulp markedly induced HGF production. There was a time lag of 72 h before induction of HGF production after the extract addition. Its stimulatory effect was accompanied by upregulation of HGF gene expression. Increases in mitogen-activated protein kinases (MAPKs) were observed from 72 h after the extract addition. Inhibitors of MAPKs suppressed the extract-induced HGF production. The extract also stimulated cell proliferation. Both activities for induction of HGF production and cell proliferation were eluted together in a single peak with 14,000 Da on gel filtration. The results indicate that bitter melon pulp extract induced HGF production and cell proliferation of human dermal fibroblasts and suggest that activation of MAPI(s is involved in the HGF induction. Our findings suggest potential usefulness of the extract for tissue regeneration and provide an insight into the molecular mechanism underlying the wound-healing property of bitter melon. (C) 2009 Elsevier Ltd. All rights reserved.