Demonstration of Mother-to-Child Transmission of Streptococcus mutans Using Multilocus Sequence Typing
Demonstration of Mother-to-Child Transmission of Streptococcus mutans Using Multilocus Sequence Typing
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DOI：
10.1159/000170588
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发表时间：
2008-01-01
期刊：
影响因子：
4.2
通讯作者：
Ooshima, T.
中科院分区：
文献类型：
--
作者：
Lapirattanakul, J.;Nakano, K.;Ooshima, T.
多位点序列分型（MLST）是一种新的可靠的基因分型方法，用于评价致龋病原体变形链球菌的垂直传播。共136个S。从20对日本母子的唾液样本中分离出变形菌菌株，其中包括5名女孩和5名男孩的乳牙列，以及5名女孩和5名男孩的混合牙列。分析了8个部分管家基因aroE、murI、gltA、glnA、glk、tkt、lepC和gyrA的核苷酸序列，并将来自母子对的菌株之间的所有这些序列的相似性视为指示传播，这在70%的对中显示。有趣的是，女孩（90%）中母亲传播菌株的比率显著高于男孩（p = 0.001）。此外，S.在每个母亲唾液样本中分布百分比最高的变异株序列类型（ST）被发现转移到其子女。此外，在两个大的共轭转移相关的区域，TnSmu1和TnSmu2的变化，进行了测定和MLST定义的ST相比。尽管来自不同家族的一些ST分离株显示出不同的TnSmu 2模式，但从具有相同ST的相同家族分离的任何菌株中，PCR模式显示这两个区域均不存在变异。我们的研究结果表明母亲是传播的主要来源。变异株，而目前的MLST方法也被证明是有用的调查细菌传播。版权所有（C）2008 S. Karger AG，巴塞尔
A new reliable genotyping method, multilocus sequence typing (MLST), was used to evaluate vertical transmission of the cariogenic pathogen Streptococcus mutans. A total of 136 S. mutans strains were isolated from saliva samples of 20 Japanese mother-child pairs, including 5 girls and 5 boys with primary dentition, and 5 girls and 5 boys with mixed dentition. The nucleotide sequences of 8 partial housekeeping genes, aroE, murI, gltA, glnA, glk, tkt, lepC, and gyrA, were analyzed and a similarity for all of those sequences between strains from a mother-child pair was regarded as indicating transmission, which was shown in 70% of the pairs. Interestingly, the rate of transmitted strains from mothers was significantly higher in the girls (90%) than in the boys (p = 0.001). Furthermore, the S. mutans sequence type (ST) with the highest distribution percentage in each maternal saliva sample was found to be transferred to their children. In addition, variations in two large conjugative-transfer associated regions, TnSmu1 and TnSmu2, were determined and compared with the STs defined by MLST. No variations in those two regions shown by PCR patterns were present in any of the strains isolated from the same families with the same STs, though isolates of some STs from different families showed distinct patterns for TnSmu2. Our results indicate that mothers are the main source for transmission of S. mutans to their children, while the present MLST method was also shown to be useful for investigating bacterial transmission. Copyright (C) 2008 S. Karger AG, Basel