17 Beta-estradiol increases endothelial nitric oxide synthase mRNA copy number in cerebral blood vessels: quantification by real-time polymerase chain reaction.
17 Beta-estradiol increases endothelial nitric oxide synthase mRNA copy number in cerebral blood vessels: quantification by real-time polymerase chain reaction.
复制标题
17 β-雌二醇增加脑血管中内皮一氧化氮合酶 mRNA 拷贝数：通过实时聚合酶链反应进行定量。
DOI：
10.1016/j.ejphar.2003.08.037
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发表时间：
2003
影响因子：
5
通讯作者：
Krause,DianaN
中科院分区：
文献类型：
--
作者：
Stirone,Chris;Chu,Yi;Sunday,Lorraine;Duckles,SueP;Krause,DianaN
内皮一氧化氮合酶（eNOS）在维持血管张力中发挥着关键作用。雌激素增强 eNOS 功能的机制仍存在争议。我们在这里使用实时聚合酶链反应 (PCR) 和免疫印迹分析证明，体内雌激素治疗导致小鼠脑血管中 eNOS 信使 RNA (mRNA) 拷贝数增加 100%，并使 eNOS 蛋白水平增加 47%。这些数据表明雌激素可以在体内血管的转录水平上调节 eNOS。
The enzyme endothelial nitric oxide synthase (eNOS) plays a critical role in the maintenance of vascular tone. The mechanism by which estrogen increases eNOS function remains controversial. We demonstrate here using real-time polymerase chain reaction (PCR) and immunoblot analysis that in vivo estrogen treatment leads to a 100% increase in eNOS messenger RNA (mRNA) copy number and increases eNOS protein levels by 47% in mouse cerebral blood vessels. These data suggest that estrogen can modulate eNOS at the transcriptional level in blood vessels in vivo.