THE EFFECTS OF A LACK OF OXYGEN AND OF LOW OXYGEN TENSIONS ON PARAMECIUM
THE EFFECTS OF A LACK OF OXYGEN AND OF LOW OXYGEN TENSIONS ON PARAMECIUM
复制标题
缺氧和低氧张力对草履虫的影响
DOI：
10.2307/1537643
复制
发表时间：
1939
期刊：
影响因子：
--
通讯作者：
J. Kitching
中科院分区：
文献类型：
--
作者：
J. Kitching
1.以多微核草履虫和尾草履虫为实验材料，分别在纯氢、纯氮和已知的氢氧混合气体中进行实验，并在显微镜下观察其在细悬液滴中的形态.在所有情况下，有一个有限的时间内无氧生存，在此期间，游泳和收缩空泡的活动逐渐减少。最后，微生物停止生长，破裂，细胞溶解。即使在水泡开始后，充足的氧气也能使病人恢复。缺氧条件下的生存是最好的培养液中保持在一个合理的氢离子浓度，通过添加少量的二氧化碳的氢或氮。在这些条件下，有些生物体存活长达12小时.草履虫在任何一种培养基中的厌氧存活时间长短都是非常可变的。这种变异性归因于生理条件，而不是遗传因素。缺氧条件下的存活时间在稀磷酸盐中要短得多。
1. Paramecium multimicronucleatum and P. caudatum were subjected to pure hydrogen, pure nitrogen, and known mixtures of hydrogen and oxygen, while in a thin hanging drop under microscopical observation.2. In all cases there was a limited period of anaerobic survival, during which activity of swimming and of contractile vacuoles was gradually diminished. Finally the organisms stopped, blistered, and cytolysed.3. Admission of sufficient oxygen, even after blistering had begun, gave recovery.4. Survival under anoxic conditions was best in culture fluid maintained at a reasonable hydrogen ion concentration by the addition of small quantities of carbon dioxide to the hydrogen or nitrogen. Under these conditions some organisms survived as long as twelve hours.5. Paramecium was extremely variable as regards the length of anaerobic survival in any one medium. This variability is ascribed to physiological condition and not to genetic factors.6. Survival under anoxic conditions was much shorter in dilute phosphate ...