A simple antigen-antibody reaction using ultrasmall FeCo nanoparticles
A simple antigen-antibody reaction using ultrasmall FeCo nanoparticles
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DOI：
10.1063/9.0000569
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发表时间：
2023-02
期刊：
影响因子：
1.6
通讯作者：
L. Tonthat;T. Murayama;N. Kobayashi;S. Yabukami;W. Ikeda-Ohtsubo;K. Arai
中科院分区：
文献类型：
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作者：
L. Tonthat;T. Murayama;N. Kobayashi;S. Yabukami;W. Ikeda-Ohtsubo;K. Arai
在这项研究中，我们制备了具有高磁矩的超小FeCo纳米颗粒（NPs），并研究了它们在生物检测中的抗原-抗体反应。在Fe:Co:Ba:F = 14:11:21:54的条件下，从feo - baf2纳米颗粒膜上收集FeCo NPs。%，由于BaF2基质潮解，通过将膜溶解在水中。FeCo NPs的尺寸为~ 5 nm，饱和磁化强度估计为~ 15.30 kG （149.0 emu/g）。采用超离心分离（11万rpm， 90 min）收集白色念珠菌抗体（abcam ab53891）结合的FeCo NPs，与白色念珠菌反应。结果表明，白色念珠菌被成功地吸收到FeCo NPs上，并且在FeCo NPs和白色念珠菌结合前在水中进行超声处理，从FeCo NPs和白色念珠菌聚集体的显微照片中可以看出，白色念珠菌的数量明显增加。高磁矩超小NPs抗原抗体反应的成功，提高了检测灵敏度，也为更小生物分子的检测提供了可能。
In this study, we prepared ultrasmall FeCo nanoparticles (NPs) with a high magnetic moment and examined their antigen-antibody reaction for biodetection applications. The FeCo NPs were collected from the FeCo-BaF2 nanogranular film with Fe:Co:Ba:F = 14:11:21:54 at.%, by dissolving the film in water since the BaF2 matrix was deliquescent. The size of FeCo NPs was ∼5 nm and the saturation magnetization was estimated to be ∼15.30 kG (149.0 emu/g). The Candida albicans antibodies (abcam ab53891)-conjugated FeCo NPs were collected by using an ultracentrifugal separation (110 000 rpm, 90 min), they were then reacted with Candida albicans. The obtained result indicates that Candida albicans were absorbed successfully onto FeCo NPs, and the number of Candida albicans bound to FeCo NPs counted from the micrographs of the aggregates of FeCo NPs and Candida albicans increased significantly by adding sonication treatment of the film in water before binding them to the antibodies. The success of antigen-antibody reaction of ultrasmall NPs with high magnetic moment improves detection sensitivity as well as offers potential detection for smaller biomolecules.