Proteomic and genetic approaches to identifying defence-related proteins in rice challenged with the fungal pathogen Rhizoctonia solani
Proteomic and genetic approaches to identifying defence-related proteins in rice challenged with the fungal pathogen Rhizoctonia solani
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DOI：
10.1111/j.1364-3703.2006.00350.x
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发表时间：
2006-09-01
影响因子：
4.9
通讯作者：
Oard, James H.
中科院分区：
文献类型：
--
作者：
Lee, Joohyun;Bricker, Terry M.;Oard, James H.
由立枯丝核菌（Rhizoctoniasolani）引起的水稻纹枯病是一种世界性的水稻主要病害，但对寄主对纹枯病的反应却知之甚少。本研究的目的是鉴定水稻抗、感锈性的蛋白质和DNA标记。索拉尼通过双向聚丙烯酰胺凝胶电泳检测接种和未接种条件下差异表达的蛋白质。使用电喷雾电离四极杆-飞行时间质谱法（ESI Q-TOF MS）进行串联质谱分析，用于使用NCBI非冗余蛋白质数据库鉴定蛋白质。在感病和抗病植株中，接种后有7种蛋白质含量增加。七个蛋白质中的六个被鉴定为具有假定的抗真菌、光合作用和蛋白水解活性。在抗性品系的反应中检测到另外14种蛋白。14个蛋白质中有11个被鉴定为与抗真菌活性、信号转导、能量代谢、光合作用、分子伴侣、蛋白水解和抗氧化有关的推测功能。首次在抗病水稻植株中检测到3-β-羟基类固醇脱氢酶/异构酶的诱导，表明该酶在水稻中对病菌的攻击具有防御作用。索拉尼四个诱导蛋白的染色体位置被发现是在物理上接近遗传标记的两个遗传作图群体中的纹枯病抗性。本研究的蛋白质组学和遗传学结果表明，水稻对稻瘟病菌攻击的反应是复杂的。solani，涉及从多个防御途径同时诱导蛋白质。
Sheath blight, caused by the fungus Rhizoctonia solani, is a major disease of rice world-wide, but little is known about the host response to infection. The objective of this study was to identify proteins and DNA markers in resistant and susceptible rice associated with response to infection by R. solani. Replicated two-dimensional polyacrylamide gel electrophoresis experiments were conducted to detect proteins differentially expressed under inoculated and non-inoculated conditions. Tandem mass spectra analysis using electrospray ionization quadrupole-time of flight mass spectrometry (ESI Q-TOF MS) was carried out for protein identification with the NCBI non-redundant protein database. Seven proteins were increased after inoculation in both susceptible and resistant plants. Six of the seven proteins were identified with presumed antifungal, photosynthetic and proteolytic activities. An additional 14 proteins were detected in the response of the resistant line. Eleven of the 14 proteins were identified with presumed functions relating to antifungal activity, signal transduction, energy metabolism, photosynthesis, molecular chaperone, proteolysis and antioxidation. The induction of 3-beta-hydroxysteroid dehydrogenase/isomerase was detected for the first time in resistant rice plants after pathogen challenge, suggesting a defensive role of this enzyme in rice against attack by R. solani. The chromosomal locations of four induced proteins were found to be in close physical proximity to genetic markers for sheath blight resistance in two genetic mapping populations. The proteomic and genetic results from this study indicate a complex response of rice to challenge by R. solani that involves simultaneous induction of proteins from multiple defence pathways.