Complete nucleotide sequence of a soybean actin gene.
Complete nucleotide sequence of a soybean actin gene.
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DOI：
10.1073/pnas.79.4.1022
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发表时间：
1982-02
影响因子：
11.1
通讯作者：
Dilip M. Shah;Robin C. Hightower;Richard B. Meagher
中科院分区：
文献类型：
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作者：
Dilip M. Shah;Robin C. Hightower;Richard B. Meagher
大豆含有一个小的肌动蛋白相关序列的多基因家族。我们测定了重组载体pSAc3上携带的大豆肌动蛋白基因的全序列。根据核苷酸序列推测，该大豆肌动蛋白由376个氨基酸组成。与其他真核生物肌动蛋白相比，pSAc3肌动蛋白在第118-122位氨基酸残基之间缺失一个氨基酸。在引发剂蛋氨酸之后是丙氨酸，在其他真核肌动蛋白的这个位置上没有丙氨酸。PSAc3肌动蛋白在初级序列上与真菌肌动蛋白和动物肌动蛋白的差异比任何已知的非植物肌动蛋白彼此之间的差异更大。在特定的NH(2)末端氨基酸的位置上，pSAc3肌动蛋白似乎与细胞质和肌肉的特异性肌动蛋白有关。编码序列被三个小内含子打断，每个内含子长度小于90个碱基对。剪接连接与其他真核基因中发现的相似，表明高等植物中存在类似的剪接装置。内含子1和3分别在密码子20和355之后中断阅读框。内含子2在第151位裂解甘氨酸密码子。这些内含子的位置都不是相对于其他被检测的肌动蛋白基因中内含子的位置保守的。
Soybean contains a small multigene family of actin-related sequences. We have determined the complete nucleotide sequence of a soybean actin gene carried on the recombinant plasmid pSAc3. As deduced from the nucleotide sequence, this soybean actin is composed of 376 amino acids. Compared to other eukaryotic actins, pSAc3 actin has a deletion of one amino acid between residues 118 and 122. The initiator methionine is followed by alanine, which is not found at this position in other eukaryotic actins. pSAc3 actin differs, in primary sequence, more from fungal and animal actins than any of the known nonplant actins differ from each other. pSAc3 actin appears to be related to both cytoplasmic and muscle specific actins in the location of specific NH(2)-terminal amino acids. The coding sequence is interrupted by three small introns, each less than 90 base pairs long. The splice junctions are similar to those found in other eukaryotic genes, suggesting the presence of a similar splicing apparatus in higher plants. Introns 1 and 3 interrupt the reading frame after codons 20 and 355, respectively. Intron 2 splits a glycine codon at position 151. None of these intron positions is conserved relative to the positions of introns in other actin genes examined.