Crystal structure of chalcone synthase, a key enzyme for isoflavonoid biosynthesis in soybean
Crystal structure of chalcone synthase, a key enzyme for isoflavonoid biosynthesis in soybean
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DOI：
10.1002/prot.25988
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发表时间：
2020-08-25
影响因子：
2.9
通讯作者：
Waki, Toshiyuki
中科院分区：
文献类型：
--
作者：
Imaizumi, Riki;Mameda, Ryo;Waki, Toshiyuki
异黄酮类化合物是类黄酮类化合物中的一种，在陆地植物的生存中起着关键作用。查尔酮合成酶（CHS）是异黄酮生物合成途径的第一个酶，催化常见异黄酮前体的形成。我们以前曾报道过，大豆CHS的同工酶（称为GmCHS1）是一个关键组成部分的大豆代谢，蛋白质复合物，以提高效率的大豆生产。在这里，我们确定了GmCHS1的晶体结构，作为了解代谢子结构的第一步，以及更好地了解GmCHS1的催化机制。
Isoflavonoid is one of the groups of flavonoids that play pivotal roles in the survival of land plants. Chalcone synthase (CHS), the first enzyme of the isoflavonoid biosynthetic pathway, catalyzes the formation of a common isoflavonoid precursor. We have previously reported that an isozyme of soybean CHS (termed GmCHS1) is a key component of the isoflavonoid metabolon, a protein complex to enhance efficiency of isoflavonoid production. Here, we determined the crystal structure of GmCHS1 as a first step of understanding the metabolon structure, as well as to better understand the catalytic mechanism of GmCHS1.