Correlative light-ion microscopy for biological applications
Correlative light-ion microscopy for biological applications
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DOI：
10.1039/c2nr30431g
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发表时间：
2012-01-01
期刊：
影响因子：
6.7
通讯作者：
Stevens, Molly M.
中科院分区：
文献类型：
--
作者：
Bertazzo, Sergio;von Erlach, Thomas;Stevens, Molly M.
在这里，我们报告了一种新的技术，相关光离子显微镜（CLIM），相关的SEM样显微照片与荧光图像。该技术与传统方法相比具有显著的优势，能够将地形和生物化学信息与纳米级分辨率相关联，而不会破坏荧光信号。我们证明了CLIM的效用，验证其潜力成为生物医学研究中的常规程序的细胞基质相互作用的各种调查。
Here we report a new technique, Correlative Light-Ion Microscopy (CLIM), to correlate SEM-like micrographs with fluorescence images. This technique presents significant advantages over conventional methods in enabling topographical and biochemical information to be correlated with nanoscale resolution without destroying the fluorescence signal. We demonstrate the utility of CLIM for a variety of investigations of cell substrate interactions validating its potential to become a routine procedure in biomedical research.