The circadian clock, light, and cryptochrome regulate feeding and metabolism in Drosophila.
The circadian clock, light, and cryptochrome regulate feeding and metabolism in Drosophila.
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DOI：
10.1177/0748730411420080
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发表时间：
2011-12
影响因子：
3.5
通讯作者：
Thummel CS
中科院分区：
文献类型：
--
作者：
Seay DJ;Thummel CS
最近对哺乳动物的研究表明，生物钟在维持代谢稳态方面发挥着核心作用。然而，尽管取得了这些进展，但人们对这些复杂途径如何协调仍知之甚少。在这里，我们证明了果蝇中新陈代谢的昼夜节律控制的基本方面是保守的。我们分析了果蝇个体的摄食行为和基本代谢水平，结果表明，与哺乳动物一样，果蝇在进食后循环糖分迅速增加，随后以糖原的形式储存。这些碳水化合物水平的日常节律在时钟突变体中被破坏，这表明生物钟在餐后进食反应中发挥着关键作用。我们还表明，基本代谢物水平以时钟依赖性方式协调，并且时钟功能是维持脂质稳态所必需的。通过检查进食行为，我们发现果蝇主要在一天的前 4 小时进食，而光线通过隐花色素光感受器抑制了晚间的进食。这些研究表明，果蝇的摄食和新陈代谢的核心方面取决于生物钟。我们的工作还揭示了光和隐花色素在摄食行为和新陈代谢中的新作用。
Recent studies in mammals have demonstrated a central role for the circadian clock in maintaining metabolic homeostasis. In spite of these advances, however, little is known about how these complex pathways are coordinated. Here, we show that fundamental aspects of the circadian control of metabolism are conserved in the fruit fly Drosophila. We assay feeding behavior and basic metabolite levels in individual flies and show that, like mammals, Drosophila display a rapid increase in circulating sugar following a meal, which is subsequently stored in the form of glycogen. These daily rhythms in carbohydrate levels are disrupted in clock mutants, demonstrating a critical role for the circadian clock in the postprandial response to feeding. We also show that basic metabolite levels are coordinated in a clock-dependent manner and that clock function is required to maintain lipid homeostasis. By examining feeding behavior, we show that flies feed primarily during the first 4 hours of the day and that light suppresses a late day feeding bout through the cryptochrome photoreceptor. These studies demonstrate that central aspects of feeding and metabolism are dependent on the circadian clock in Drosophila. Our work also uncovers novel roles for light and cryptochrome on both feeding behavior and metabolism.