Antidepressant-like effect of active fraction of Polyrhachisvicina Roger in a rat depression model
Antidepressant-like effect of active fraction of Polyrhachisvicina Roger in a rat depression model
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Polyrhachisvicina Roger 活性部位在大鼠抑郁模型中的抗抑郁样作用
DOI：
10.1016/j.jtcm.2018.02.001
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发表时间：
2018
影响因子：
2.6
通讯作者：
Chen Naihong
中科院分区：
文献类型：
--
作者：
Wei Guining;Chu Shifeng;Su Qibiao;Su Hua;Lin Meiyu;He Fei;Lu Wenjie;Lu Guoshou;Huang Zhoufeng;Tan Xiao;Lin Xiao;Zeng Xianbiao;Wei Baowei;Chen Naihong
目的：研究拟黑多刺蚁有效部位(AFPR)对抑郁模型大鼠的抗抑郁作用，并探讨其作用机制。方法：采用乙醇-石油醚提取法。采用小鼠悬尾实验(TST)、强迫游泳实验(FST)和旷场实验(OPT)观察其抗抑郁药样作用。采用利血平重复给药(0.5 mg/kg，每日1次，连续14d)建立大鼠抑郁模型。以氟西汀为阳性对照。观察AFPR对利血平诱导的大鼠上睑下垂、体温过低和运动不能的影响，以及对河马校园和前额叶皮质单胺类物质及其代谢产物含量和单胺氧化酶(MAO)活性的影响。结果：160 mg/kg和320 mg/kg的AFPR灌胃给药可显著缩短FST和TST的不动时间，对OPT的运动能力无明显影响。在利血平诱导的抑郁模型中，AFPR通过逆转体温过低、动作迟缓和蔗糖消耗来减轻快感障碍。AFPR可显著增加多巴胺、5-羟色胺、去甲肾上腺素和乙酰胆碱等单胺类物质的浓度。结论：AFPR可使去甲肾上腺素、5-羟色胺和多巴胺的代谢率及MAO活性恢复正常，这可能是利血平慢性暴露引起的。这些发现表明，单胺能神经递质系统的调节可能是AFPR抗抑郁药样作用的基础。(C)2018年JTcm。版权所有。
OBJECTIVE: To investigate the antidepressant-like effect of active fraction of Polyrhachis vicina Roger (AFPR) in a rat depression model, and to elucidate the underlying mechanism. METHODS: AFPR was extracted with ethanol followed by petroleum ether. Its antidepressant-like effect was investigated in mice by tail suspension test (TST), forced swimming test (FST) and open field test (OPT). A repeated dose of reserpine (0.5 mg/kg, daily for 14 d) was used to establish a rat depression model. Fluoxetine was used as positive control agent. The effect of AFPR on reserpine-induced ptosis, hypothermia and akinesia, the levels of monoamines and their metabolites, and the activity of monoamine oxidase (MAO) in hippo campus and prefrontal cortex were determined. RESULTS: Administration of AFPR by gavage at 160 and 320 mg/kg significantly reduced the duration of immobility in the FST and TST, and did not affect locomotor activity in the OPT. In the reserpine-induced depression model, AFPR attenuated anhedonia, demonstrated by reversing hypothermia, akinesia and sucrose consumption. AFPR significantly increased the concentration of monoamines, including dopamine, serotonin, noradrenaline and acetylcholine. CONCLUSION: AFPR normalized the metabolism rates of noradrenaline, serotonin and dopamine, and the activity of MAO, which were altered by chronic reserpine exposure. The findings suggest that modulation of the monoaminergic neurotransmitter system likely underlies the antidepressant-like effect of AFPR. (C) 2018 JTCM. All rights reserved.