Haploid accumulation and translational control of phosphoglycerate kinase-2 messenger RNA during mouse spermatogenesis.
Haploid accumulation and translational control of phosphoglycerate kinase-2 messenger RNA during mouse spermatogenesis.
复制标题
小鼠精子发生过程中磷酸甘油酸激酶 2 信使 RNA 的单倍体积累和翻译控制。
DOI：
10.1016/0012-1606(83)90368-8
复制
发表时间：
1983
影响因子：
2.7
通讯作者：
Hecht,NB
中科院分区：
文献类型：
--
作者：
Gold,B;Fujimoto,H;Kramer,JM;Erickson,RP;Hecht,NB
磷酸甘油酸激酶-2（PGK-2）的睾丸特异性同工酶mRNA的细胞内定位已被确定为两种生精细胞类型。从粗线期初级精母细胞或圆形精子细胞的多聚体和非多聚体组分的PGK-2的mRNA活性已通过无细胞翻译与多肽产物通过免疫沉淀监测，随后通过一维或二维电泳和荧光法测定。结果表明，圆形精子细胞PGK-2 mRNA的活性主要存在于多聚体组分中，而粗线期初级精母细胞PGK-2 mRNA的活性相对较低，存在于非多聚体组分中。在原始A型精原细胞或青春期前支持细胞的胞质RNA中未观察到PGK-2 mRNA活性。这些数据表明，成熟的PGK-2 mRNA首先出现在减数分裂前期的生精细胞的细胞质中，并在减数分裂后的数量增加。虽然成熟的PGK-2 mRNA存在于减数分裂细胞中，但它直到减数分裂完成后才被主动翻译。因此，mRNA的积累和翻译机制参与了精子发生过程中磷酸甘油酸激酶-2合成的控制。
The intracellular location of the mRNA for the testis-specific isozyme of phosphoglycerate kinase-2 (PGK-2) has been determined for two spermatogenic cell types. The mRNA activity for PGK-2 from the polysomal and nonpolysomal fractions of pachytene primary spermatocytes or round spermatids has been assayed by cell-free translation with the polypeptide products monitored by immunoprecipitation, followed by one-dimensional or two-dimensional electrophoresis and fluorography. The results reveal that the majority of PGK-2 mRNA activity of round spermatids was present in the polysomal fraction while the relatively less abundant PGK-2 mRNA of pachytene primary spermatocytes was present in the nonpolysomal fraction. No PGK-2 mRNA activity was observed in the cytoplasmic RNA from primitive type A spermatogonia or prepubertal Sertoli cells. These data indicate that mature PGK-2 mRNA first appears in the cytoplasm of spermatogenic cells during the prophase of meiosis and increases in amount after meiosis. Although mature PGK-2 mRNA is present in meiotic cells it is not actively translated until after meiosis has been completed. Thus, mRNA accumulation and translational mechanisms are involved in the control of phosphoglycerate kinase-2 synthesis during spermatogenesis.