Biogenic amines, caffeine and tonic immobility in Tribolium castaneum
Biogenic amines, caffeine and tonic immobility in Tribolium castaneum
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DOI：
10.1016/j.jinsphys.2010.01.002
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发表时间：
2010-06-01
影响因子：
2.2
通讯作者：
Miyatake, Takahisa
中科院分区：
文献类型：
--
作者：
Nishi, Yusuke;Sasaki, Ken;Miyatake, Takahisa
生物胺是一种生理神经活性物质，影响无脊椎动物的行为和生理特征。本文研究了多巴胺、章鱼胺、酪胺和5-羟色胺对卡氏拟谷盗紧张性静止或死亡伪装的影响。这些胺被注射到人工选择的甲虫的腹部，以保持长时间或短时间的紧张不动。在长品系甲虫中，注射多巴胺、章鱼胺和酪胺可缩短紧张性不动的持续时间，且这些胺的作用呈剂量依赖关系。另一方面，注射5-羟色胺并不影响紧张性静止的持续时间。在很少假装死亡的短应变甲虫中，没有发现胺对紧张性不动的持续时间有显著影响。章鱼胺、酪胺和5-羟色胺的大脑表达水平在长品系甲虫和短品系甲虫之间没有差异，而此前报道的短品系甲虫的多巴胺水平更高。在口服吸收和注射实验中，咖啡因都缩短了假装死亡的时间。众所周知，咖啡因能激活多巴胺。因此，目前的结果表明，卡氏毛滴虫的紧张性不动持续时间是通过多巴胺受体影响的。(C)2010爱思唯尔有限公司。保留所有权利。
Biogenic amines are physiologically neuroactive substances that affect behavioural and physiological traits in invertebrates. In the present study, the effects of dopamine, octopamine, tyramine and serotonin on tonic immobility, or death-feigning, were investigated in Tribolium castaneum. These amines were injected into the abdomens of beetles artificially selected for long or short duration of tonic immobility. In beetles of the long strains, the durations of tonic immobility were shortened by injection of dopamine, octopamine and tyramine, and the effects of these amines were dose-dependent. On the other hand, serotonin injection did not affect the duration of tonic immobility. In the short-strain beetles that rarely feign death, no significant effects of the amines were found on the duration of tonic immobility. Brain expression levels of octopamine, tyramine and serotonin did not differ between long- and short-strain beetles, in contrast to the higher dopamine levels in short strains previously reported. Caffeine decreased the duration of death-feigning in both oral absorption and injection experiments. It is known that caffeine activates dopamine. Therefore, the present results suggest that the duration of tonic immobility is affected by dopamine via the dopamine receptor in T. castaneum. (C) 2010 Elsevier Ltd. All rights reserved.