Functional analysis of mRNA 3' end formation signals in the convergent and overlapping transcription units of the S. cerevisiae genes RHO1 and MRP2.
Functional analysis of mRNA 3' end formation signals in the convergent and overlapping transcription units of the S. cerevisiae genes RHO1 and MRP2.
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酿酒酵母基因 RHO1 和 MRP2 的会聚和重叠转录单元中 mRNA 3 末端形成信号的功能分析。
DOI：
10.1093/nar/21.23.5500
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发表时间：
1993
影响因子：
14.9
通讯作者：
Myers,AM
中科院分区：
文献类型：
--
作者：
Peterson,JA;Myers,AM
酿酒酵母RHO1和MRP2是聚合转录的，其终止密码子之间有281个碱基对分隔。转录作图显示 RHO1-MRP2 编码区内至少有 111 个碱基对在两个方向上转录。置换实验表明，MRP2 的 70 nt 序列和 RH01 的 179 nt 序列足以形成正常的 mRNA 3' 末端。 MRP2 信号在任一方向上都起作用，尽管在非自然方向上效率相对较低。该元件包含两个方向的 3' 末端形成所必需的 PolyAT 序列。RH01 或 MRP23' 末端形成不受互补的天然反义转录物的过量产生或消除的影响。相反，插入一个将反义转录物延伸到其正常3'末端之外的强启动子会使MRP2或RH01失活。这些数据表明，紧凑酵母基因组中的转录终止对于防止反义转录导致下游基因失活非常重要。
TheSaccharomyces cerevisiaegenesRHO1andMRP2are convergently transcribed, with 281 base pairs separating their termination codons. Transcript mapping revealed at least 111 base pairs within theRHO1-MRP2intercoding region are transcribed in both directions. Transplacement experiments showed distinct sequences of 70 nt forMRP2and 179 nt forRH01were sufficient for normal mRNA 3' end formation. TheMRP2signal functioned in either orientation, although relatively inefficiently in the nonnative orientation. This element contains a polyAT sequence essential for 3' end formation in both orientations.RH01orMRP23' end formation was not affected by overproduction or elimination of the complementary, natural antisense transcript. In contrast, insertion of a strong promoter that extended antisense transcripts beyond their normal 3' ends inactivated eitherMRP2orRH01. These data suggest that transcript termination in the compact yeast genome can be important to prevent inactivation of downstream genes as a result of antisense transcription.