Evaluation of Risk Factors for Peste des Petits Ruminants Virus in Sheep and Goats at the Wildlife-Livestock Interface in Punjab Province, Pakistan
Evaluation of Risk Factors for Peste des Petits Ruminants Virus in Sheep and Goats at the Wildlife-Livestock Interface in Punjab Province, Pakistan
复制标题
DOI：
10.1155/2016/7826245
复制
发表时间：
2016-01-01
影响因子：
--
通讯作者：
Wensman, Jonas Johansson
中科院分区：
文献类型：
--
作者：
Aziz-ul-Rahman;Abubakar, Muhammad;Wensman, Jonas Johansson
小反胃害虫病毒(PPRV)是巴基斯坦家畜和野生小型反刍动物的一种高发病率、高死亡率的传染病，造成了巨大的经济损失。本研究旨在探讨野生动物园附近家养小型反刍动物感染猪繁殖与呼吸综合征病毒的危险因素。从两个不同的野生动物园采集了265份血清样本(27份野生反刍动物和238份家养小型反刍动物)，并对其进行了PPRV抗体检测。此外，还收集了20只有呼吸道症状的家养小型反刍动物的鼻拭子，以检查是否存在PPRV抗原。竞争酶联免疫吸附试验显示，在拉合尔野生动物园周围的家养小型反刍动物中，抗PPRV抗体的比例最高(35%)，而在费萨拉巴德(13%)，这些公园的野生反刍动物的测试血清中不存在PPRV抗体。雌性(25.6%)高于雄性(5.1%)，山羊(34.5%)高于绵羊(11.2%)。基于N基因的RT-PCR结果突出表明，由于在研究期间该地区没有发生当前的PPR疫情，因此没有出现PPRV。尽管放牧不是一个重要的风险因素，但对于饲料和蓄水池来说，仍然存在野生动物-牲畜相互作用的可能性，导致PPR对野生动物的溢出。鉴于PPR的高血清反应性和高风险，应采取疫苗接种，以避免PPRV在野生和家养小型反刍动物(绵羊和山羊)中传播。
Peste des petits ruminants virus (PPRV) is causing infectious disease with high morbidity and mortality rate in domestic and wild small ruminants of Pakistan with valuable economical losses. The present study was carried out to investigate risk factors of PPRV in domestic small ruminants which were present in the vicinity of wildlife parks. A total of 265 sera samples (27 wild ruminants and 238 domesticated small ruminants) from apparently healthy animals from two different wildlife parks were collected and analysed for PPRV antibodies. Also, 20 nasal swabs from domestic small ruminants showing respiratory signs were collected to check for presence of PPRV antigen. Competitive ELISA revealed highest proportions of anti-PPRV antibodies in domestic small ruminants around the Wildlife Park at Lahore (35%) as compared to Faisalabad (13%), with no existence of PPRV antibodies in tested serum of wild ruminants at these parks. Higher seropositivity was observed in females (25.6%) than in males (5.1%) and in goats (34.5%) compared to sheep (11.2%). The results of N-gene based RT-PCR highlight the absence of PPRV due to lack of current PPR outbreak in the region during study period. Even though grazing was not a significant risk factor, there is still a possibility of wildlife-livestock interactions for feed and water reservoirs, resulting in spillover of PPR to wildlife. Keeping in view the high seropositivity and risk of PPR, vaccination should be adopted to avoid circulation of PPRV among wild and domestic small ruminants (sheep and goats).