Targeted Near-Infrared Fluorescent Turn-on Nanoprobe for Activatable Imaging and Effective Phototherapy of Cancer Cells
Targeted Near-Infrared Fluorescent Turn-on Nanoprobe for Activatable Imaging and Effective Phototherapy of Cancer Cells
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用于癌细胞可激活成像和有效光疗的靶向近红外荧光开启纳米探针
DOI：
10.1021/acsami.5b02037
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发表时间：
2016-06-22
影响因子：
9.5
通讯作者：
Han, Heyou
中科院分区：
文献类型：
--
作者：
Li, Na;Li, Tingting;Han, Heyou
一种新型的绿色多功能纳米平台作为药物输送，细胞成像和光治疗的纳米载体已被工程化。该纳米平台由作为核的稳定碳球（CS）、包覆的聚多巴胺（PDA）壳、靶向叶酸（FA）和负载的抗癌药物吲哚菁绿色（ICG）组成，获得CS @PDA-FA@ICG纳米复合物（NC）。生物相容的PDA壳提供了高的荧光猝灭效率和富含用于锚定FA以靶向癌细胞的官能团的表面。通过疏水作用和π-π-堆积，芳香族ICG可以有效地负载到CSs@PDAFA体系中，负载效率为58.9%。特别是激活的NIR。细胞内环境中的荧光使CS @PDA-FA@ICG成为可用于NIR成像的敏感的“OFF”到“ON”纳米探针。此外，与单独的ICG相比，CSs@PDA-FA@ ICG NC可以在单一NIR激光照射下诱导用于同时协同光动力学疗法（PDT）和光热疗法（PTT）的有效光转化。结果表明，CSs@PDA-FA@ICG NC作为靶向和活化的纳米平台，为促进癌症的准确诊断和增强治疗效果提供了新的机会。这项工作激发了更多的兴趣，在设计的简易表面功能化策略，以构建其他多功能纳米复合材料，如纳米管和纳米棒。
A novel and green multifunctional nanoplatform as a nanocarrier for drug delivery, cell imaging, and photo therapy has been engineered. The nanoplatform is composed of stabilized carbon spheres (CSs) as cores, a coated polydopamine (PDA) shell, targeted folic acid (FA), and the loaded anticancer drug indocyanine green (ICG), obtaining CSs@PDA-FA@ICG nanocomposites (NCs). The biocompatible PDA shell provided a high fluorescence quenching efficiency and a surface rich in functional groups for anchoring FA for targeting cancer cells. Aromatic ICG could be effectively loaded into the CSs@PDAFA system via hydrophobic interactions and pi-pi-stacking with a loading efficiency of 58.9%. Notably, the activated NIR. fluorescence in an intracellular environment made CSs@PDA-FA@ICG a sensitive "OFF" to "ON" nanoprobe that can be used for NIR imaging. Moreover,, compared to ICG alone, the CSs@PDA-FA@ ICG NCs could induce efficient photoconversion for simultaneous synergetic photodynamic therapy (PDT) and photothermal therapy (PTT) under a single NIR laser irradiation. The results demonstrated that CSs@PDA-FA@ICG NCs as a targeted and activated nanoplatform provide new opportunities to facilitate the accurate diagnosis of cancer and enhanced treatment efficacy. This work stimulates more interest in the design of the facile surface functionalization strategy to construct other multifunctional nanocomposites, such as nanotubes and nanorods.