Characterization of allosteric insulin hexamers by electrospray ionization mass spectrometry.
Characterization of allosteric insulin hexamers by electrospray ionization mass spectrometry.
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通过电喷雾电离质谱法表征变构胰岛素六聚体。
DOI：
10.1021/ac980753s
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发表时间：
1999
影响因子：
7.4
通讯作者：
Fenselau,C
中科院分区：
文献类型：
--
作者：
Fabris,D;Fenselau,C
在pH 8.0下使用电喷雾质谱法，结合准确的质量测量，以确认与Zn（II）离子形成稳定非共价复合物的胰岛素的多样性。参与Zn（II）螯合的配体的性质和数量的测定与晶体和溶液结构一致。计算参与每个中心的配体的数量允许推导配体场的几何构型，并给出关于溶液中胰岛素单体的构象状态的间接信息。
Electrospray mass spectrometry is used at pH 8.0 in combination with accurate mass measurements to confirm the multiplicity of insulin in stable noncovalent complexes with Zn(II) ions. Determination of the nature and number of ligands involved in Zn(II) chelation is in agreement with crystal and solution structures. Counting the number of ligands participating in each center allows deduction of the geometric configuration of the ligand field and gives indirect information about the conformational state of the insulin monomers in solution.